Re: [Histonet] Histonet Digest, Vol 247, Issue 4

2024-06-09 Thread Izak Dimenstein via Histonet
What about Rene J. Buesa Histology without Xyline. Annals of Diagnostic Pathology, 05 Feb 2009, 13(4):246-256 https://doi.org/10.1016/j.anndiagpath.2008.12.005 PMID: 19608083 Share this article Share with emailShare with twitterShare with linkedinShare with facebook _

Re: [Histonet] Histonet Digest, Vol 245, Issue 20

2024-05-01 Thread Tony Henwood via Histonet
al at Westmead (Retired) Adjunct Fellow, School of Medicine, University of Western Sydney. From: John O’Brien via Histonet Sent: 01 May 2024 01:49 To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Histonet Digest, Vol 245, Issue 20 Ethyl Alcohol, Reagen

Re: [Histonet] Histonet Digest, Vol 245, Issue 20

2024-04-30 Thread John O’Brien via Histonet
Ethyl Alcohol, Reagent 100% alcohol is what is normally used in Pathology processing and slide staining IMEB Inc offer all grades of Reagent alcohol, pure ethyl is a controlled alcohol regulated and taxed by government Regards John > On Apr 30, 2024, at 10:18 AM, histonet-requ...@lists.utso

Re: [Histonet] Histonet Digest, Vol 245, Issue 17

2024-04-25 Thread Bob Richmond via Histonet
Ann Specian (where?) asks about Epredia Xylene Substitute: >>We are thinking of changing from xylene to this substitute. Does anybody have any Processing protocols using Epredia Xylene?substitute that they could share?<< Well, what’s in it? You’re asking your readers to look it up for you. I

Re: [Histonet] Histonet Digest, Vol 245, Issue 17

2024-04-25 Thread John O’Brien via Histonet
Very slow drying of all substitutes > On Apr 25, 2024, at 10:11 AM, histonet-requ...@lists.utsouthwestern.edu wrote: > > Send Histonet mailing list submissions to >histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit >http://lists.utsouthweste

Re: [Histonet] Histonet Digest, Vol 245, Issue 9

2024-04-12 Thread dogsloveus2 via Histonet
Hi Pam! Just curious about the position in Salem, Virginia.  Is it a permanent position?  Do you have details on it?   Thanks!  Tara Yahoo Mail: Search, Organize, Conquer On Fri, Apr 12, 2024 at 12:11 PM, histonet-requ...@lists.utsouthwestern.edu wrote: Send Histonet mailing list submiss

Re: [Histonet] Histonet Digest, Vol 243, Issue 7

2024-02-09 Thread Bob Richmond via Histonet
> > Re: [Histonet] Modified Davidson > > The formula for "modified Davidson's fixative" that I used was three parts tap water, three parts reagent alcohol, two parts 37% formaldehyde ("strong formalin"), one part glacial acetic acid. Best mixed under a fume hood. At 85 I must be one of the last le

Re: [Histonet] Histonet Digest, Vol 243, Issue 6

2024-02-08 Thread Paula Keene Pierce via Histonet
hanie Thompson - 210-428-1646 > > > > This message contains privileged and confidential information intended only > for the use of the addressee named above. If you are not the intended > recipient of this message you must not disseminate, copy, or take any action > in reliance on it. > > > -- > > Subject: Digest Footer > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > -- > > End of Histonet Digest, Vol 243, Issue 6 > ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Histonet Digest, Vol 243, Issue 6

2024-02-08 Thread KATHLEEN FERNANDEZ via Histonet
Paula, We are using the KP’s at our lab too and have been noticing that they are smearing! We are having issues on our slides, since we hand write still. This just recently started happening. Maybe they changed their formula? Kathy Sent from my iPhone > On Feb 8, 2024, at 12:01 PM, histonet-req

Re: [Histonet] Histonet Digest, Vol 242, Issue 10

2024-01-25 Thread Izak Dimenstein via Histonet
I would like to add a couple cents to Brian's suggestions, especially how to embed the gross section. The "theory" in presented in my book Izak Dimenstein Grossing Bones: Principles, Technique, and Instruments. It depends on the level of hardness of the bone after decalcification or in the case

Re: [Histonet] Histonet Digest, Vol 237, Issue 4

2023-09-20 Thread Eddie Martin via Histonet
r Avenue, MC 1-283 > Houston, TX 77030 > > Office: 832-355-6524 | Fax: 832-355-6812 > Email: bmolin...@texasheart.org > texasheart.org<https://www.texasheart.org/> | texasheartmedical.org< > https://www.texasheartmedical.org/> | facebook< > https://www.facebook.com/Te

Re: [Histonet] Histonet Digest, Vol 235, Issue 23

2023-06-28 Thread Pat Patterson via Histonet
Get Outlook for Android From: histonet-requ...@lists.utsouthwestern.edu Sent: Wednesday, June 28, 2023 12:00:02 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 235, Issue 23 [CAUTION - EXTERNAL EMAIL: Do not clic

Re: [Histonet] Histonet Digest, Vol 235, Issue 13

2023-06-17 Thread Brent Barrett via Histonet
Unsubscribe From: histonet-requ...@lists.utsouthwestern.edu Sent: Saturday, June 17, 2023 1:00:02 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 235, Issue 13 Send Histonet mailing list submissions to histonet@lists.utsouthwestern

Re: [Histonet] Histonet responses

2023-06-13 Thread Mac Donald, Jennifer via Histonet
Hi Anthony, If you hit reply only you will get a response. If you hit reply all a response will go you and the Histonet. Jennifer -Original Message- From: Anthony Lapiana via Histonet Sent: Tuesday, June 13, 2023 11:58 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet

[Histonet] Histonet responses

2023-06-13 Thread Anthony Lapiana via Histonet
Good afternoon, I am new to the histonet and I am trying to figure out how to reply to people's messages they post. Also, do I get a notification when people reply to my responses or posts via email? Thanks, Anthony ___ Histonet mailing list Histonet@l

Re: [Histonet] Histonet Digest, Vol 235, Issue 1

2023-06-08 Thread Janice Fuller via Histonet
Is there fresh tissue in that part of the lab? Or anything else that would cause it to be considered not clean? On Fri, Jun 2, 2023 at 10:00 AM wrote: > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web,

Re: [Histonet] Histonet Digest, Vol 234, Issue 10

2023-05-10 Thread Patsy Ruegg via Histonet
Aren't they going to offend the mail histotechs with that name? Just kidding. Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 39037 N 11th Ave Phoenix, AZ 85086 C 720-281-5406 prueg...@hotmail.com Doug Ruegg C720-281-5407 douglas...@hotmail.com From: histonet-requ

Re: [Histonet] Histonet Digest, Vol 233, Issue 7

2023-04-19 Thread Patsy Ruegg via Histonet
this is a strange post on histonet Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 39037 N 11th Ave Phoenix, AZ 85086 C 720-281-5406 prueg...@hotmail.com Doug Ruegg C720-281-5407 douglas...@hotmail.com From: histonet-requ...@lists.utsouthwestern.edu Sent: Wednesd

Re: [Histonet] Histonet Digest, Vol 233, Issue 3

2023-04-07 Thread John O’Brien via Histonet
Need pictures of microtome and blade holder to help IMEB Inc > On Apr 7, 2023, at 10:17 AM, histonet-requ...@lists.utsouthwestern.edu wrote: > > Send Histonet mailing list submissions to >histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit >ht

Re: [Histonet] Histonet Digest, Vol 232, Issue 13

2023-03-23 Thread Ewen Sutherland via Histonet
Re Gunk Check any of the seals or washers. When they breakdown they usually present with this sort of result on slides Ewen Sutherland Anatomical and Digital Product Specialist Bio-Strategy T: +61 3 9355 3900 / 1800 00 84 53 M: +61 417 460 019 ewen.sutherl...@bio-strategy.com   www.bio-strateg

Re: [Histonet] Histonet Digest, Vol 232, Issue 7

2023-03-22 Thread Eddie Martin via Histonet
sitive. He couldn’t figure out why after > several tries to reduce background. Can someone give me some suggestions? > Thank you very much! > > > Ken > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu >

Re: [Histonet] Histonet Digest, Vol 232, Issue 11

2023-03-21 Thread Izak Dimenstein via Histonet
>From my Grossing Technology book: Gouty tissue contains monosodium urate crystals. Tissue specimens with gout diagnosis must be submitted in either Carnoy’s fixative (chloroform, acetic acid, and absolute ethanol, now almost abandoned in regular practice) or in absolute ethanol. Fixation canno

Re: [Histonet] Histonet Digest, Vol 231, Issue 14

2023-02-27 Thread Izak Dimenstein via Histonet
Placenta is a serious surgical pathology specimen. Wrong initial processing can damage the result. The described processing in the laboratory reflects complete misunderstanding by the management the grossing procedure. In my book Grossing Technology (Amazon.com), placenta grossing technique occ

Re: [Histonet] Histonet Digest, Vol 229, Issue 10

2022-12-28 Thread John O’Brien via Histonet
IMEB Inc Has complete catalog of all dermatology and pathology supplies and equipment new and refurbished for last 35 years www.imebinc.com IMEB Inc Management > On Dec 28, 2022, at 10:08 AM, histonet-requ...@lists.utsouthwestern.edu wrote: > > Send Histonet mailing list submissions to >hi

Re: [Histonet] Histonet Digest, Vol 228, Issue 13

2022-12-04 Thread John O’Brien via Histonet
Hi Chris My company has large selection of refurbished and slightly used Leica cryostats all models, clinical and research Please contact me for complete list and details ,all instruments come complete with full warranty Regards John IMEB Inc www.imebinc.com > On Nov 23, 2022, at 10:11 AM, hi

Re: [Histonet] Histonet Digest, Vol 228, Issue 9

2022-11-21 Thread Kathy Sherwood via Histonet
ads were very good today. Very cold out . I'll talk to you later. I'll be thinking of you. Much love, Kathy From: histonet-requ...@lists.utsouthwestern.edu Sent: Thursday, November 17, 2022 1:00 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest,

Re: [Histonet] Histonet Digest, Vol 228, Issue 3

2022-11-04 Thread Katie Riley via Histonet
Hello Cindy, I am looking for an auto stainer and microtome. Please contact me if you have either. Thank you, Katie Riley-Hamilton H.T., M.T.QA Technical Supervisor of Dermatopathology Puyallup Dermatology Clinic ka...@puyallupderm.com 2622 South Meridian Puyallup, WA 98373 (253) 266-0799 (25

Re: [Histonet] Histonet Digest, Vol 225, Issue 14

2022-09-06 Thread Eddie Martin via Histonet
re > osteochondral grafts, but I did not find the information I was looking for. > Thank you in advance > Best wishes > > Alida > > -- > Dr. Alida M. Bailleul > Associate Professor > Institute of Vertebrate Paleontology and Paleoanthro

Re: [Histonet] Histonet Digest, Vol 226, Issue 1

2022-09-01 Thread kidist Shamebo via Histonet
sclosure, dissemination, distribution or copying of > it or its contents is prohibited. If you have received this communication > in error, please reply to the sender immediately or by telephone at > 413-794- and destroy all copies of this communication and a

Re: [Histonet] Histonet Digest, Vol 225, Issue 5--REMOVE FROM LIST

2022-08-08 Thread Jill Simmonds via Histonet
Please remove me from subscription list. Thanks, Jill Jill Simmonds | Account Executive Clinical and Research Reagents Phone: 925.586.6268 | www.biocare.net Biocare Medical, LLC 60 Berry Drive, Pacheco CA 94553 This electronic transmission (and any attached document) contains information from

Re: [Histonet] Histonet Digest, Vol 225, Issue 5

2022-08-06 Thread John O’Brien via Histonet
To whom it concerns Please contact IMEB Inc for info on refurbished Leica Slide or cassette printer we been selling refurbishing Leica slide and cassette printers for 15 years, As long as u have software and stand alone unit I have refurbished printers with complete warranty of 9 months On t

Re: [Histonet] Histonet Digest, Vol 225, Issue 1

2022-08-03 Thread Joseph Brooks via Histonet
; > > Today's Topics: > >1. Small processors (Anne Murvosh) > > > -- > > Message: 1 > Date: Tue, 2 Aug 2022 15:23:37 + > From: Anne Murvosh > To: "histonet (histonet@lists.utsouthwestern.edu)" > > Subject: [Histonet] Small processors > Mes

Re: [Histonet] Histonet Digest, Vol 225, Issue 1

2022-08-02 Thread Rob Rankin via Histonet
cessors (Anne Murvosh) > > > -- > > Message: 1 > Date: Tue, 2 Aug 2022 15:23:37 + > From: Anne Murvosh > To: "histonet (histonet@lists.utsouthwestern.edu)" > > Subject: [Histon

Re: [Histonet] Histonet Digest, Vol 224, Issue 9

2022-07-24 Thread John O’Brien via Histonet
Tom I agree about Dr.McCormick Regards John IMEB May he Rest In Peace > On Jul 24, 2022, at 10:09 AM, histonet-requ...@lists.utsouthwestern.edu wrote: > > Send Histonet mailing list submissions to >histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, vi

Re: [Histonet] Histonet Digest, Vol 223, Issue 14

2022-06-24 Thread Patsy Ruegg via Histonet
I used tonsil as + control. Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 39037 N 11th Ave Phoenix, AZ 85086 C 720-281-5406 prueg...@hotmail.com Doug Ruegg C720-281-5407 douglas...@hotmail.com From: histonet-requ...@lists.utsouthwestern.edu Sent: Friday, June 2

Re: [Histonet] Histonet Digest, Vol 220, Issue 8

2022-03-17 Thread Tony Henwood (SCHN) via Histonet
il.com] Sent: Friday, 18 March 2022 12:45 AM To: Tony Henwood (SCHN) Subject: Re: [Histonet] Histonet Digest, Vol 220, Issue 8 Hello Henwood, Thank you for the reply to my post with elaborate explanation and appropriate references. We doubted about the validity of using after expiry date. We ke

Re: [Histonet] Histonet Digest, Vol 220, Issue 8

2022-03-17 Thread Cartun, Richard via Histonet
sts.utsouthwestern.edu] Sent: Wednesday, March 16, 2022 9:18 PM To: jayalakshmy p.s Cc: 'histonet@lists.utsouthwestern.edu' Subject: Re: [Histonet] Histonet Digest, Vol 220, Issue 8 EXTERNAL email from Outside HHC! Do NOT open attachments or click links from unknown senders. Hi Jaya

Re: [Histonet] Histonet Digest, Vol 220, Issue 8

2022-03-16 Thread Tony Henwood (SCHN) via Histonet
thwestern.edu] Sent: Thursday, 10 March 2022 1:27 PM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Histonet Digest, Vol 220, Issue 8 Hello all, I would like to know whether Immunohistochemistry markers can be used after its expiry date(atleast in resource poor countries). If yes, f

Re: [Histonet] Histonet Digest, Vol 220, Issue 8

2022-03-09 Thread jayalakshmy p.s via Histonet
com/> > > > > Secured by Paubox - HITRUST CSF certified > https://www.paubox.com > ---- > > > > -- Forwarded message -- > From: John Garratt > To: Nancy Schmitt , " > histonet@lists.utsouthwestern.edu" > Cc: > Bcc: > Da

Re: [Histonet] Histonet Digest, Vol 216, Issue 5

2021-11-13 Thread Linda Hines via Histonet
me for embedding the tissue samples goes directly > from 70% alcohol to the embedding step in paraffin. > > > Correct me if I am wrong but shouldn't the tissue be dehydrated fully and > cleared before embedding the samples? > ___

Re: [Histonet] Histonet Digest, Vol 215, Issue 18

2021-10-25 Thread Kathryn Perkinson via Histonet
Open Position: Laboratory Supervisor Histology which is part of the Division of Anatomic Pathology and Digital Analytics. This position offers an opportunity to supervise a large laboratory with 3 shifts and weekends. It is part of a larger division which includes IHC, FISH, and Digital Imagin

Re: [Histonet] Histonet Digest, Vol 215, Issue 3

2021-10-05 Thread John Frazier via Histonet
[Histonet] FW: Biopsy processing > > > From: Hannen, Valerie > Sent: Monday, October 04, 2021 2:54 PM > To: 'histo...@list.utsouthwestern.edu' > Subject: FW: Biopsy processing > > > > From: Hannen, Valerie > Sent: Monday, October 4, 2021 11:03 AM > To: 'histo...@list.utsouthwestern.edu' > Subject: Biopsy processing > > Good Morning All... My Pathologist is wondering if there is any > benefit to having a separate processing run just for small biopsies? He > said that what is seeing now, he has no issue with, the sections are just > as good as all the other tissue sections. He just asked for me to get the > consensus of the group. > > Thank You in advance!! > > > > > Valerie A. Hannen,MLT(ASCP),HTL,SU(FL) > Histology Section Chief > Parrish Medical Center > 951 N. Washington Avenue > Titusville, Florida 32796 > P: 321-268-6333 Ext. 7506 > F: 321-268-6149 > valerie.han...@parrishmed.com<mailto:valerie.han...@parrishmed.com> > www.parrishmed.com<http://www.parrishmed.com> > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Histonet Digest, Vol 214, Issue 14

2021-09-28 Thread Kathryn Perkinson via Histonet
se.com/v3/__https://www.arrayscience.com/publications*Posters__;Iw!!OToaGQ!6kZFsSGmGAqtHSUCXGvBt6QPRHlQc7P4geZI9L6HlrVmY_FPmCDYnGHGszj4cR01HGjCd-A$ Best regards, Regan Regan Fulton, M.D., Ph.D. CEO and Co-Founder Array Science, LLC 475 Gate 5 Road, #100 Sausalito, CA 94965 (415) 577-7360 email

Re: [Histonet] Histonet Digest, Vol 214, Issue 1

2021-09-01 Thread John O’Brien via Histonet
) > 2. Anyone Using LaserTrack PH6 or PH8 Tissue CassettePrinter > General Data (Donna Emge) > > > -- > > Message: 1 > Date: Tue, 31 Aug 2021 17:08:45 + > From: Patsy Ruegg > To:

Re: [Histonet] Histonet Digest, Vol 213, Issue 22

2021-08-31 Thread Patsy Ruegg via Histonet
I would suggest using something like histogel or agar to make layers of blood drops in a tube, then freeze them and make frozen sections. Sounds tricky. Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 39037 N 11th Ave Phoenix, AZ 85086 C 720-281-5406 prueg...@hotmail.com Doug Ruegg C720-281-5407 d

Re: [Histonet] Histonet Digest, Vol 213, Issue 6

2021-08-09 Thread Wooten, Jennifer via Histonet
We just recently purchased the Epredia NX50, which we use with our older HM525s. We love it, I just wish they would make height adjustability a standard option on them! Jennifer Wooten, BA, BS, HTL (ASCP)CM Pronouns: she/her/hers Technical Supervisor | Anatomic Pathology | University Hospital je

Re: [Histonet] Histonet Digest, Vol 212, Issue 14

2021-07-27 Thread Izak Dimenstein via Histonet
In contrast to gynecological cytology, nongynecological cytology (NGYN) does not have a separate CPT code for screening. Details in my book's Procedural Coding in Anatomic Pathology Cytopathology section, pp. 162-176. Izak Dimenstein From: histonet-requ...@lists.u

Re: [Histonet] Histonet Digest, Vol 212, Issue 7

2021-07-16 Thread richard.wild--- via Histonet
Message: 1 Date: Wed, 14 Jul 2021 22:03:24 + (UTC) From: Cheryl To: "histonet@lists.utsouthwestern.edu" Subject: [Histonet] PathCentre (ThermoShandon) protocols - help? Message-ID: <7393119.276682.1626300204...@mail.yahoo.com> Content-Type: text/plain; charset=UTF-8 Help?? Starti

Re: [Histonet] Histonet Digest, Vol 212, Issue 1

2021-07-06 Thread Wooten, Jennifer via Histonet
We surface decal with decal I for about 20-30 minutes (as long as you don't need to do any molecular studies). You can usually get 3-4 good sections before its crunchy again. Jennifer Wooten, BA, BS, HTL (ASCP)CM Pronouns: she/her/hers Technical Supervisor | Anatomic Pathology | University Hospi

Re: [Histonet] Histonet Digest, Vol 211, Issue 7

2021-06-07 Thread Patsy Ruegg via Histonet
The blue stain from prussian blue is a chemical reaction and indicates that there is a lot of iron in the section, you would not want it not to stain some of the iron there would you? I agree with John, take an adjacent section for a general stain. Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting

Re: [Histonet] Histonet Digest, Vol 208, Issue 14 Best Slides for Leica IP S Slide Printers

2021-03-27 Thread Steve McClain via Histonet
Be careful. Look at the slides before you commit. The least expensive may also be the dirtiest. So stack up 12 slides and look through them. If they look cloudy or dirty. Nix Do the drop test. A slide should be able to survive being dropped to the floor from waist high. Choose on price after

Re: [Histonet] Histonet Digest, Vol 208, Issue 4

2021-03-06 Thread Ashwin Ashwin via Histonet
Hello Everyone, PM me for Histo positions in Florida Thanks Ashwin -Original Message- From: histonet-requ...@lists.utsouthwestern.edu Sent: Saturday, March 6, 2021 1:00 PM To: histonet@lists.utsouthwestern.edu Subject: {EXTERNAL} Histonet Digest, Vol 208, Issue 4 CAUTION! This email

Re: [Histonet] Histonet Digest, Vol 208, Issue 1

2021-03-01 Thread John Frazier via Histonet
ent(s). It may > contain confidential or proprietary information and may be subject to > attorney-client privilege or other confidentiality protections. If you are > not a designated recipient you may not review, copy or distribute this > message. If you receive this in error, pl

Re: [Histonet] Histonet Digest, Vol 207, Issue 7

2021-02-10 Thread John Frazier via Histonet
stonet@lists.utsouthwestern.edu > Bcc: > Date: Tue, 9 Feb 2021 15:21:26 -0500 > Subject: Re: [Histonet] H&E Stainers > Dako has one > > Sent from my iPhone > > > On Feb 9, 2021, at 12:25 PM, raestask wrote: > > > > Is there a stainer other than Ventana

Re: [Histonet] Histonet Digest, Vol 207, Issue 3 Bouin's vs Vinegar

2021-02-05 Thread Steve McClain via Histonet
Date: Thu, 4 Feb 2021 22:36:26 + From: Luigi Mascio To: "histonet@lists.utsouthwestern.edu" Subject: Re: [Histonet] Histonet Digest, Vol 207, Issue 2 Message-ID: Content-Type: text/plain; charset="iso-8859-1" Buy Bouin's from a consumable

Re: [Histonet] Histonet Digest, Vol 207, Issue 2

2021-02-04 Thread Luigi Mascio via Histonet
Buy Bouin's from a consumable company and pour it in your own spray bottle! Luigi M. Mascio BS HT (ASCP) Technical Director lu...@medequipsource.com 724-687-7342 direct number 724-369-1604 ext. 102 412-216-0496 cell For a great resource visit medequipsource.com, especially our blog with histol

Re: [Histonet] Histonet Digest, Vol 206, Issue 14

2021-01-27 Thread Olszewski, Dawn via Histonet
We use the red bag method after we pull out any we want to use as controls and after Oncology pulls out any they want for Tumor bank. Dawn Olszewski Pathology Manager - Histotechnologist Laboratory [cid:MasterLogo_190x61_362365a5-c941-4f16-9f99-9484fd6a6078.png] South Georgia Medical Center 25

Re: [Histonet] Histonet Digest, Vol 206, Issue 14

2021-01-27 Thread John Frazier via Histonet
hank you :) > > Melissa Kuhnla > Lead Medical Technologist > for IHC and FISH testing > Regional Laboratory Services > Good Samaritan Hospital > 631-609-2551 > > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.u

Re: [Histonet] Histonet Digest, Vol 206, Issue 1 New Tissue Processor? (Sandra Cheasty)

2021-01-08 Thread Steve McClain via Histonet
While (conceptually) looking forward to the virtues of new and improved modern tissue processors w WiFi and SMS messaging capability, I fully expect to be operating the 1979 K-series for years to come. I reckon we may be behind the times, but with regular annual maintenance, our VIP5 and even

Re: [Histonet] Histonet Digest, Vol 205, Issue 2

2020-12-06 Thread Izak Dimenstein via Histonet
In my experience and opinion, grossing and embedding are the main sources of extraneous tissues ("floaters"). This issue is discussed in detail in the section "Root Cause Analysis for floaters prevention during grossing and embedding in surgical pathology histology laboratory" in my book Grossin

Re: [Histonet] Histonet Digest, Vol 204, Issue 6

2020-11-13 Thread Krista Ranalli via Histonet
ns to adhere better throughout the process? > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Histonet Digest, Vol 203, Issue 21

2020-10-29 Thread Eddie Martin via Histonet
d is 15,000-20,000 slides/year. > > Many thanks in advance for your help. > > Kind regards > W.A Hassan > > > > > -- Forwarded message -- > From: Tina Van Meter > To: warda hassan > Cc: Histonet@lists.utsouthwestern.edu > Bcc: > Date: We

Re: [Histonet] Histonet Digest - Nikon NIS Elements

2020-08-28 Thread Jennifer White via Histonet
Amy, I have been using Nikon's NIS Elements D imaging software for several years along with a Nikon DS-Fi1 digital camera to capture images of various histological slides, as well as small whole-mount specimens on a dissecting scope. I have been happy with its ease of use and the images that it

Re: [Histonet] Histonet Digest, Vol 201, Issue 15

2020-08-22 Thread Izak Dimenstein via Histonet
The rotator cuff/joint study is an interesting project. I wish I had an encounter with such a specimen before I’d written Grossing Bones: Principles, Techniques and Instruments book (Amazon.com). The problem is not in fixation, or even in a saw, but rather in getting the initial slab/s which pre

Re: [Histonet] Histonet Digest, Vol 201, Issue 3 Update info. on old topic?

2020-08-05 Thread Steve McClain via Histonet
Tim, Paula and Jeanine I have little experience w slide scanning, yet I have captured nearly 1.8M slide images, mostly on film coverslipped slides. One year ago we switched back to glass. 1) I suspect what Tim describes may be due to the fact that the refractive index of film differs from glass.

Re: [Histonet] Histonet Digest, Vol 201, Issue 3

2020-08-05 Thread Madeleine Huey via Histonet
Sanders, Jeanine (CDC/DDID/NCEZID/DHCPP)" > Cc: Histonet > Subject: Re: [Histonet] Update info. on old topic? > Message-ID: > < > byapr05mb57039195dfe1e6c67b67b1f7e7...@byapr05mb5703.namprd05.prod.outlook.com > > > > Content-Type: text/plain; charset=&quo

Re: [Histonet] Histonet Digest, Vol 200, Issue 19

2020-07-30 Thread Madeleine Huey via Histonet
Original Message- From: Ken M via Histonet Sent: Tuesday, July 28, 2020 11:43 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Apple Green Birefringence in Amyliod slides Hi Ken, May I give you my personal experience on Congo Red? 1) Always cut thick tissue (ie,10um thickness, but

Re: [Histonet] Histonet Digest, Vol 200, Issue 9

2020-07-10 Thread Ken Urban via Histonet
Charles Culling is the name/person you’re trying to remember. Sent from my iPhone > On Jul 10, 2020, at 12:00 PM, histonet-requ...@lists.utsouthwestern.edu wrote: > > Send Histonet mailing list submissions to >histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World

Re: [Histonet] Histonet Digest, Vol 200, Issue 5

2020-07-08 Thread Muhammad Azam via Histonet
;iso-8859-1" > > Prof. Kiernan, as usual, provides us all with such a depth/breadth of > particular information/advice. > His Histological and Histochemical methods BIBLE is still my favourite read. > __

Re: [Histonet] Histonet Digest, Vol 200, Issue 5

2020-07-08 Thread King,Michael A via Histonet
Happy to second that opinion, that was probably the single most valuable training and reference text in my career as a research neurobiologist (also hunt down his archived Histonet posts on formaldehyde fixation, essential reading for lab pros). Others apparently agreed, as I had to replace a co

Re: [Histonet] Histonet Digest, Vol 199, Issue 12

2020-06-23 Thread Dee Neamand via Histonet
Hi Anne. We’ve been recycling xylene for many years. We purchased our unit through CBG biotech. They have a formalin recycling unit and a solvent recycling unit. The solvent unit can be used to recycle ethanol, xylene, and xylene substitutes. Let me know if you need more info or would like to ch

Re: [Histonet] Histonet Digest, Vol 199, Issue 9

2020-06-16 Thread Madeleine Huey via Histonet
Hello, Does anyone have Covid 19 FFPE tissues or Transient/Stable Cell Line (slides/block) ? I have some H.Pylori blocks for exchanges. *Madeleine Huey, B.S., HTL & QIHC (ASCP)* Supervisor, Pathology/Histology & IPOX (MV & LG) El Camino Hospital 2500 Grant Road (Rm GC-33) Mountain View, CA 94

Re: [Histonet] Histonet Digest, Vol 198, Issue 1

2020-05-03 Thread King,Michael A via Histonet
Make sure sodium azide (antimicrobial preservative) is not used in any of your IHC reagents--it inhibits peroxidase. Mike --- Message: 3 Date: Fri, 1 May 2020 08:00:49 -0700 From: Kristy Castillo To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC DAB Message-ID: Conte

Re: [Histonet] Histonet Digest, Vol 197, Issue 23 recovering RNA from FFPE

2020-04-25 Thread Paula Keene Pierce via Histonet
631 361-4000 cell 631 926-3655 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

Re: [Histonet] Histonet Digest, Vol 197, Issue 23 recovering RNA from FFPE

2020-04-25 Thread Paula Keene Pierce via Histonet
11787 631 361-4000 cell 631 926-3655 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet

Re: [Histonet] Histonet Digest, Vol 197, Issue 23 recovering RNA from FFPE

2020-04-25 Thread Steve McClain via Histonet
1) Does anyone know of a method of recovering RNA from FFPE? 2) Does anyone know a lab that has done it? Thanks, Steve A. McClain, MD McClain Labs 45 Manor Road Smithtown, NY 11787 631 361-4000 cell 631 926-3655 ___ Histonet mailing list Histonet@lists.

Re: [Histonet] Histonet Digest, Vol 197, New Topic Coronavirus testing from tissue sample

2020-04-23 Thread Steve McClain via Histonet
The CDC website with specimen submission form does not seem to be working. https://www.cdc.gov/laboratory/specimen-submission/pdf/form-50-34.pdf Does anyone know who can else can test for Corona virus from FFPE tissue? I have an unusual skin biopsy from late December (earlier than the first known

Re: [Histonet] Histonet Digest, Vol 197, Issue 8

2020-04-14 Thread John Garratt via Histonet
---- > > Message: 2 > Date: Thu, 9 Apr 2020 11:15:47 -0400 > From: "Pam Barker" rel...@earthlink.net > To: "Histopeeps

Re: [Histonet] Histonet Digest, Vol 197, Issue 8

2020-04-14 Thread Dionee Neamand via Histonet
Hello. I am looking for help with IHC staining. Does anyone have good negative control tissue for MMR stains? We need a minimum of 10 negative results per IHC stain for our validation. We have yet to find any. Any suggestions would be helpful. Thanks,Dee Dionee Neamand, HT (ASCP)Lead TechEvangel

Re: [Histonet] Histonet Digest, Vol 197, Issue 11

2020-04-13 Thread Izak Dimenstein via Histonet
> Today's Topics: > > 1. Re: Question concerning H. pylori staining (jkier...@uwo.ca) > > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _

Re: [Histonet] Histonet Digest, Vol 196, Issue 3 Incomplete sectioning

2020-03-04 Thread Steve McClain via Histonet
rom your computer. > Thank you. > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@li

Re: [Histonet] Histonet Digest, Vol 196, Issue 3

2020-03-04 Thread NWL - Histology via Histonet
Re: Histonet digest Incomplete sectioning of blocks. I had to manage this situation a few year back and found that the best place to capture this is at the waterbath. QC reviews I.e numbers of return slides categorised into fault type can be collated over a month and displayed easily to a team

Re: [Histonet] Histonet Digest, Vol 195, Issue 10

2020-02-24 Thread White, Marcia via Histonet
We do not assist but do go in and perform a frozen section, if this is the assistance they are giving the pathologist then this is normal Marcia White Director Memorial Regional Pathology Services 9581 Premier Parkway, Miramar FL 33025 (954) 276-1855 | Fax: 954-967-7627     -Original Mes

Re: [Histonet] Histonet Digest, Vol 195, Issue 10

2020-02-14 Thread Patsy Ruegg via Histonet
I never minded being called in to assist for an aspect of transplants at the U, I figured I was helping a very needy patient. Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 prueg...@hotmail.com

Re: [Histonet] Histonet Digest, Vol 195, Issue 6

2020-02-09 Thread Karim Ruknuddin via Histonet
Dear All, I am planning to introduce RNAscope in my pathology core facility, we are currently gathering information on both chromogen detection and florescent detection, but the company we are looking into for reagent ordering which is ACDbio has a wide range of channel 1 and channel 2 probes o

Re: [Histonet] Histonet Digest, Vol 195, Issue 1

2020-02-04 Thread LEROY H BROWN via Histonet
Hi, I am looking for a decent Gram +/- control tissue. Would be happy to exchange another type of control in exchange for a good block of tissue for this. I have great PAS or Warthan Starry or ? others too.Can someone help with this? Thanks. LeRoy Brown HT(ASCP) HTL 1...@comcast.net is m

Re: [Histonet] Histonet Digest, Vol 194, Issue 15

2020-01-21 Thread Betsy Molinari via Histonet
...@texasheart.org texasheart.org | facebook | twitter -Original Message- From: Rathborne, Toni Sent: Tuesday, January 21, 2020 8:56 AM To: Betsy Molinari Subject: RE: [Histonet] Histonet Digest, Vol 194, Issue 15 *** Important*** This email is not from Texas Heart Institute. Only click links or

Re: [Histonet] Histonet Digest, Vol 194, Issue 15

2020-01-21 Thread Betsy Molinari via Histonet
To: Betsy Molinari Subject: Re: [Histonet] Histonet Digest, Vol 194, Issue 15 *** Important*** This email is not from Texas Heart Institute. Only click links or open attachments you know are safe. I have never done this before but I would assume you could reseal it ju

Re: [Histonet] Histonet Digest, Vol 194, Issue 15

2020-01-21 Thread Betsy Molinari via Histonet
-Original Message- From: Amos Brooks via Histonet Sent: Monday, January 20, 2020 5:59 PM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Histonet Digest, Vol 194, Issue 15 *** Important*** This email is not from Texas Heart Institute. Only click links or open attachments you know

Re: [Histonet] Histonet Digest, Vol 194, Issue 15

2020-01-20 Thread Amos Brooks via Histonet
Hi Betsy, I wouldn't do it. It's an unnecessary risk to let them dry out. Better to leave it in distilled water if you absolutely must. Ideal to not deparaffinize in the first place if you can't finish the stain. Dried out sections is just a bad plan. Amos Brooks On Mon, Jan 20, 2020, 1:00 P

Re: [Histonet] Histonet Digest, Vol 194, Issue 12btattoo removal

2020-01-17 Thread Steve McClain via Histonet
I don not know any method for tattoo pigment removal. Aside from carbon black, most of the red, yellow, blue and green pigments are metal salts and polarize brightly. The inflammatory response may be vigorous, especially w repeat or Re-do of a tattoo. In some cases a pseudo-carcinoma or KA may

Re: [Histonet] Histonet Digest, Vol 194, Issue 10

2020-01-15 Thread LEROY H BROWN via Histonet
How do I sign up one of my employees to be given NSH membership. What is the cost? Thanks, LeRoy Brown HT(ASCP) HTL -Original Message- From: histonet-requ...@lists.utsouthwestern.edu Sent: Wednesday, January 15, 2020 10:00 AM To: histonet@lists.utsouthwestern.edu Subject: Histonet Dige

Re: [Histonet] Histonet Digest, Vol 194, Issue 7

2020-01-12 Thread LEROY H BROWN via Histonet
I am looking for a small sample of normal stomach for my IHC stain control. I am willing to trade any of my control blocks if you have a block you can spare. Thanks, LeRoy Brown HT(ASCP) HTL -Original Message- From: histonet-requ...@lists.utsouthwestern.edu Sent: Sunday, January 12, 202

Re: [Histonet] Histonet Digest, Vol 194, Issue 2

2020-01-03 Thread Olszewski, Dawn via Histonet
Charles, We use the same protocol but we also cut everything at 5 microns. Dawn Olszewski HTL(ASCP)QIHC Pathology Manager South Georgia Medical Center P: (229) 259-4830 E: dawn.olszew...@sgmc.org Dawn Olszewski Pathology Manager - Histotechnologist Laboratory [cid:MasterLogo_190x61_362365a

Re: [Histonet] Histonet Digest, Vol 192, Issue 4

2019-11-06 Thread Muhammad Azam via Histonet
ific > than "Re: Contents of Histonet digest..." > ___ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Histonet Digest, Vol 192, Issue 4

2019-11-06 Thread Samira Seif via Histonet
To whom it may concern, I would like to stop getting email from histonet. I was wondering if you let me know how can I do that. Regards. Samira Sent from Mail for Windows 10 From: histonet-requ...@lists.utsouthweste

Re: [Histonet] Histonet Digest, Vol 191, Issue 12

2019-10-20 Thread Subash Govender via Histonet
6813 -- Message: 2 Date: Fri, 18 Oct 2019 23:00:16 + From: Subash Govender To: "histonet@lists.utsouthwestern.edu" Subject: Re: [Histonet] Histonet Digest, Vol 191, Issue 10 Message-ID: Content-Type: text/plain; charset="Windows-1252" Re:

Re: [Histonet] Histonet Digest, Vol 191, Issue 11

2019-10-18 Thread Subash Govender via Histonet
RE:How to prevent bubbling in pressure cooker for immunohistochemistry procedure. Hi there Mark. Really appreciate your feedback. I do use tween 20 in the retrieval solution but actually thought it was to clean the slides, thus preventing background staining. I did not know it prevents bubble

Re: [Histonet] Histonet Digest, Vol 191, Issue 10

2019-10-18 Thread Subash Govender via Histonet
t;UTF-8" Hi Atsoka! I?d be willing to trade you fresh slides for those foggy ones- studying effects of age on charges slides. Contact me at: Dave.c.juliano at gmail dot com if you?re interested! On Thu, Oct 17, 2019 at 8:24 AM Atoska Gentry via Histonet < h

[Histonet] Histonet] Freezing spray in cryostats

2019-09-26 Thread White, Lisa M. via Histonet
We did use freeze spray in the cryostat years ago. Then one fateful day we all had to go on protocol for exposure to TB because a resident sprayed a lung with the freeze spray and there you go. There is a turn around time for frozen sections we are all working with, HOWEVER it is not worth the

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