Hi kdea...@hotmail.com, What likely is occurring but you didn’t mention in the thread is that your friend is using alkaline phosphatase based detection, which, by itself can react with kidney tissue. This likely explains why your friend is getting strong kidney staining and weak melan-a staining.
This also tells me your friend didn’t optimize the antibody correctly. I’m not sure why your friend is choosing to use a lyophilized antibody for Melan-A as so many other commercially available options are available capable of refrigerated storage and good up to 3 years. CellMarque makes an Melan-A in both realty to use and/or a liquid concentrate with a 3-year expiry date. Best wishes, Eddie Martin, HTL,QIHC The National Institutes of Health Department of Laboratory Medicine Bone Marrow Service eddie.mar...@nih.gov (301)-594-2054 On Wed, Mar 15, 2023 at 1:00 PM <histonet-requ...@lists.utsouthwestern.edu> wrote: > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-requ...@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-ow...@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > Today's Topics: > > 1. Problems with Melan A staining (Ken M) > > > > ---------- Forwarded message ---------- > From: Ken M <kdea...@hotmail.com> > To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu > > > Cc: > Bcc: > Date: Wed, 15 Mar 2023 16:51:03 +0000 > Subject: [Histonet] Problems with Melan A staining > One of my histotech friends is having issues with Melan A staining. Using > MA5-27949 Melan A antibody from Thermofisher, the negative Kidney tissue > control was stained strongly positive while the positive melanoma tissue > control was stained weakly positive. He couldn’t figure out why after > several tries to reduce background. Can someone give me some suggestions? > Thank you very much! > > > Ken > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
