We are having a discussion on how long to keep the slides for IHC validation?
As long as you have the instrument? Or can the slides be disposed of at some
given time (5years?)?
Thank you for your input-
Nancy Schmitt MLT, HT(ASCP)
Histology Coordinator
United Clinical Laboratories
visit us at w
-5107
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-------- Original Message
Subject: [Histonet] How long ...
From: Massimo <[1]max_histo...@yah= oo.it>
Date: Sun, September 19, 2010 3:41 pm
To: [2]histo...@lists.uts= outhwestern.edu
Cc: [3]histo...@lists.uts= outhwestern.edu
Hi all,
I fo
I found into a shelf of the laboratory a small flask containing mouse samples
fixed in Bouin's fluid and preserved in ethanol at 70°, forgotten there for a
few years.
I wonder if it would be possible to continue the process up to paraffin
embedding for histological preparations.
Some time ago a
Hi all,
I found into a shelf of the laboratory a small flask containing mouse samples
fixed in Bouin's fluid and preserved in ethanol at 70°, forgotten there for a
few years.
I wonder if it would be possible to continue the process up to paraffin
embedding for histological preparations.
Some ti
Never let them air dry! The staining won't work.
They can stay in water quite some time, like hours. I even think they
can stay 2-3 days at 4 degrees, but I'm not sure...
With the buffers be careful! They can't stay in the buffers containing
permeabilyzing agent (like tween20 or triton) longe
How long can you leave deparaffinized slides in water or buffer if you want to
do IHC staining? Is leaving them in water or buffer preferable o letting them
air dry? I was taught to never let sections dry out.
Kimberly C. Tuttle HT (ASCP)
Pathology Biorepository and Research Core
University o
