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Date: Thu, Nov 26, 2009 10:26 PM
To: "Histonet@lists.utsouthwestern.edu";
Subject: [Histonet] IBA1-microglia staining on frozen sections
Hi all, i just can not get this IBA1 (marker of microglia) well stained on my
frozen sections fixed with acetone (sections
Hi all, i just can not get this IBA1 (marker of microglia) well stained on my
frozen sections fixed with acetone (sections were either dried up in air for 1
hour or fixed immediately). But I always get good staining on PFA-perfused
brain tissue.
Will IBA1 antigen reactivity lose in acetone fix
