The primary antibody is the most expensive reagent
If you use the primary ab unconjugated and visualise using a conjugated
secondary you will significantly increase the dilution factor of the primary ab
thus your primary ab will last much longer ( saving you money..and, maybe
less background
If you want a stronger signal I would probably use the secondary antibody vs
using a directly conjugated CD11c.
You could use a directly conjugated one as you would for flow cytometry but it
won't give as much amplification as it would with a secondary step built in.
Katy
-Original Message
