Hello, I have a question that what is best way to block endougenous biotin and HRP in liver or kidney tissue. The method I am using has to use avidin-biotin and HRP reactions. This is fine on heart tissue. I used biotin blocking kit from DAKO or Vector. Also I used 1% H2O2. But liver tissue still show background stain. Could you teach me a better way to block or is there other reason cause background? Thanks in advance, Amy
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