m: jshea...@roadrunner.com
> To: histonet@lists.utsouthwestern.edu
> Date: Thu, 19 May 2011 21:11:01 -0400
> Subject: [Histonet] IHC pos. & neg. control question
>
> Curt is right, according to CAP ...
>
> ANP.22570 QC - Antibodies Phase II
>
> Appropriate negative controls are
, Colorado 80504
-Original Message-
From: Amos Brooks [mailto:amosbro...@gmail.com]
Sent: Friday, May 20, 2011 3:05 PM
To: Liz Chlipala
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] IHC pos. & neg. control question
Thanks Liz,
You are absolutly right there, but have
Behalf Of Amos
> Brooks
> Sent: Friday, May 20, 2011 2:16 PM
> To: jm.lapoi...@accellab.com; histonet@lists.utsouthwestern.edu
> Subject: [Histonet] IHC pos. & neg. control question
>
> Hi,
> This is simply a question of definitions. They are actually both
> right.
>
Friday Folks,
Amos
Message: 5
Date: Thu, 19 May 2011 13:25:49 -0400
From: "Jean-Martin Lapointe"
Subject: [Histonet] IHC pos. & neg. control question
To:
Message-ID:
<
befd613bd39142499989f836556ddc8301272...@ace.accellab.lan>
Content-Type: text/plain; charset=&
os
Message: 5
Date: Thu, 19 May 2011 13:25:49 -0400
From: "Jean-Martin Lapointe"
Subject: [Histonet] IHC pos. & neg. control question
To:
Message-ID:
<
befd613bd39142499989f836556ddc8301272...@ace.accellab.lan>
Content-Type: text/plain; charset="iso-8859-1&
l.com]
Sent: Friday, May 20, 2011 11:57 AM
To: Curt Tague
Cc: Ingles Claire; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] IHC pos. & neg. control question
Curt,
Thank you. I'm here all week, try the veal.
Sincerely,
Curt,
Thank you. I'm here all week, try the veal.
Sincerely,
Jay A. Lundgren, M.S., HTL (ASCP)
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwester
AM
To: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] IHC pos. & neg. control question
And the block in question has already been proven positive using THAT
procedure and antibody during validation.
Claire
From: histonet-boun...@lists.utsouthwestern.edu
; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] IHC pos. & neg. control question
Pete,
When you run a positive control. The tissue is already a known positive
(or it should be) for whichever antibody you are running regardless of
prior handling. It would be impossible for this no
: [Histonet] IHC pos. & neg. control question
Pete,
When you run a positive control. The tissue is already a known positive
(or it should be) for whichever antibody you are running regardless of
prior handling. It would be impossible for this not to be so. However,
with a negative, the con
essage-
From: pete.peder...@healthonecares.com [mailto:pete.peder...@healthonecares.com]
Sent: Thursday, May 19, 2011 2:31 PM
To: Dawson, Glen; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] IHC pos. & neg. control question
Glen,
If I am to understand you correctly you are saying con
ailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jean-Martin
Lapointe
Sent: Friday, 20 May 2011 7:50 AM
To: Angela Bitting; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] IHC pos. & neg. control question
I can certainly agree with that. Whether it’s the patient’s sample or the known
po
ailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
pete.peder...@healthonecares.com
Sent: Friday, 20 May 2011 5:31 AM
To: gdaw...@dynacaremilwaukee.com; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] IHC pos. & neg. control question
Glen,
If I am to understand you correc
Curt is right, according to CAP ...
ANP.22570 QC - Antibodies Phase II
Appropriate negative controls are used.
NOTE: Negative controls must assess the presence of nonspecific staining in
patient tissue as well
as the specificity of each antibody. Results of controls must be documented,
either
.@healthonecares.com
[mailto:pete.peder...@healthonecares.com]
Sent: Thursday, May 19, 2011 2:54 PM
To: Thomas Jasper; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] IHC pos. & neg. control question
Thomas,
Agreed, however, how can you say with certainty that the control is
still good,
...@copc.net]
Sent: Thursday, May 19, 2011 1:39 PM
To: Pedersen Pete; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] IHC pos. & neg. control question
Pete,
When you run a positive control. The tissue is already a known positive
(or it should be) for whichever antibody you are run
: Thursday, May 19, 2011 4:50 PM
To: Jean-Martin Lapointe; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] IHC pos. & neg. control question
I agree with the majority. A patient slide stained without primary antibody,
and a patient section with primary antibody and a known positive con
ean-Martin
--
Message: 11
Date: Thu, 19 May 2011 13:37:38 -0500
From: "Sebree Linda A"
Subject: RE: [Histonet] IHC pos. & neg. control question
To: "Dawson, Glen" ,
Message-ID:
Content-Type: text/plain; charset=&quo
...@dynacaremilwaukee.com; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] IHC pos. & neg. control question
Glen,
If I am to understand you correctly you are saying control tissue is not
treated the same as patient tissue, therefore is useless as a negative
control correct? Then inversely doesn&#x
awson, Glen
Sent: Thursday, May 19, 2011 12:32 PM
To: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] IHC pos. & neg. control question
IMHO: Running any piece of tissue as a control that does not belong to the
patient being tested makes zero sense. Because it would not be from the
I agree 100% with Glen.
Jan Shivers
UMN VDL
- Original Message -
From: "Dawson, Glen"
To:
Sent: Thursday, May 19, 2011 1:32 PM
Subject: RE: [Histonet] IHC pos. & neg. control question
IMHO: Running any piece of tissue as a control that does not belong to the
patien
y 19, 2011 1:32 PM
To: histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] IHC pos. & neg. control question
IMHO: Running any piece of tissue as a control that does not belong to
the patient being tested makes zero sense. Because it would not be from
the patient tissue being tested, ho
sage-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Curt
Tague
Sent: Thursday, May 19, 2011 11:04 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] IHC pos. & neg. control question
I got this email from a pathologist t
9, 2011 12:04 PM
Subject: [Histonet] IHC pos. & neg. control question
I got this email from a pathologist today. we have always run a positive
with the patient tissue and a negative, the same patient tissue, and had no
problems. Am I missing something. Is there any documented regulation
dictat
] Im Auftrag von
Jean-Martin Lapointe
Gesendet: Donnerstag, 19. Mai 2011 19:26
An: histonet@lists.utsouthwestern.edu
Betreff: [Histonet] IHC pos. & neg. control question
Hi Curt,
I agree with your pathologist. The section that you use as a negative
control (without primary) in an IHC run sh
ionel-Bertrand, Boisbriand
Québec, Canada J7H 1N8
tel: 450-435-9482 ext.247
fax: 450-435-4795
jm.lapoi...@accellab.com
--
Message: 24
Date: Thu, 19 May 2011 09:04:24 -0700
From: "Curt Tague"
Subject: [Histonet] IHC pos. & neg. control quest
Curt,
Nonsense. The negative control is used to evaluate endogenous staining
in the *patient* tissue. Your Pathologist needs to do another residency at
clown college.
Sincerely,
Jay A. Lundgren, M.S., HTL (ASCP)
__
stern.edu] On Behalf Of Curt
Tague
Sent: Thursday, May 19, 2011 11:04 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] IHC pos. & neg. control question
I got this email from a pathologist today. we have always run a positive
with the patient tissue and a negative, the same patient ti
I got this email from a pathologist today. we have always run a positive
with the patient tissue and a negative, the same patient tissue, and had no
problems. Am I missing something. Is there any documented regulation
dictating what needs to be used for the controls. In some cases if we get
one sli
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