Hello,
Would anyone be willing to share their protocol for performing iron stains on
bone marrow crush specimens? We are trying to convert from doing the stain
manually to automated on a Ventana Special Stainer using the iron stain kit
from Roche. We are using the following protocol:
1.
ern.edu]
Sent: Wednesday, 8 August 2018 9:34 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Iron Stain
Is nuclear fast red the only counter stain for Prussian blue stain? I have a
Masson's trichrome kit and was wondering if the Bierbech Scarlet could be a
counterstain. I won't
HI Tasha,
The nuclear fast red will stain the nuclei red, the biebrich scarlet is a
connective tissue stain and may cover up the (sometimes sparse) prussian
blue localization of iron in the tissues. I, personally, would not consider
these reagents swappable.
In my experience nuclear fast red does
Oops, yes, I am mixing up neutral red - and the recipe from Bryan is what I
use - it last forever.
I have had luck with nuclear fast red from vector - comes made up. That
lasts a little longer than lab made.
mills
ᐧ
On Wed, Aug 8, 2018 at 12:52 PM Caroline Miller wrote:
> HI Tasha,
>
> The nuc
1% neutral red in 0.01% acetic acid (1 mL of 1% per 100 mL stain) works,
as does 1% safranin. Both are stable, although should be filtered from
time to time. Stain 1 minute, water rinse, ethanol, xylene and mount
should work. I had one pathologist who preferred a very light
progressive H&E. She
Tasha Campbell, B.S.,HTL(ASCP), at Frederick Gastroenterology Associates in
Frederick, Maryland asks:
>>Is nuclear fast red the only counter stain for the Prussian blue stain? I
have a Masson's trichrome kit and was wondering if [Biebrich] scarlet could
be [used as] a counterstain. I won't be doin
Is nuclear fast red the only counter stain for Prussian blue stain? I have a
Masson's trichrome kit and was wondering if the Bierbech Scarlet could be a
counterstain. I won't be doing the iron stain very often at all so I am trying
to keep dry reagents on hand to make up as needed so they do n
ts.utsouthwestern.edu'"
Sent: Monday, November 12, 2012 2:31 PM
Subject: [Histonet] Iron Stain
Hi All,
Is there any specific reason why one iron stain kit I have recommends that
you air-dry the bone marrow and blood smears after the Fe stain is completed
and then coverslip them while
Hi All,
Is there any specific reason why one iron stain kit I have recommends that
you air-dry the bone marrow and blood smears after the Fe stain is completed
and then coverslip them while other kits don't mention that at all. I have
always stained my smears then dehydrate, clear and cover
Our iron stain (Newcomer kit) works great for tissue sections but is not
adequate for identifying sideroblasts in a bone marrow film.
What is the preferred iron stain to identify sideroblasts and which company do
you purchase the kit from? Thank you.
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