Dear Histonetters Hi. Now I am doing immuno-stain of the cells from bronchioalveolar lavage(BAL). The primary antibody is for detecting some intracellular antigen(antigen in cytoplasm). So I think permeabilization process may be necessary after fixation by 4% PFA. Does anyone know whether we can use Tween-20 in place of Triton-100 for permeabilization? And can we use same protocol in immunostaining of BAL cells as in immunostaining for cell line?
Thanks in advance. H Kimura _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet