Hello all, Yes, the Leica Bond and Bond Max has an Alk Phos detection kit available. Though the red can bleed if left in alcohol, it is more stable than other branded red detection kits. The Red kit is also very stable for long term storage and exceedingly better than the competitor brands that also provide an automated IHC solution.
In lieu of dehydrating, consider drying them in an oven post rinse to avoid the dehydration steps prior to clearing and mounting. Cheers, Eddie Martin The National Institutes of Health Bone marrow pathology section Bethesda, MD 20892 On Fri, Jun 10, 2022 at 1:00 PM <histonet-requ...@lists.utsouthwestern.edu> wrote: > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-requ...@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-ow...@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > Today's Topics: > > 1. Red chroamgen/LeicaBond andBondMax (John O?Brien) > 2. Re: Red chroamgen/LeicaBond andBondMax (Tim Morken) > 3. Re: Red chroamgen/LeicaBond andBondMax (Cartun, Richard) > > > > ---------- Forwarded message ---------- > From: "John O’Brien" <j...@imebinc.com> > To: histonet@lists.utsouthwestern.edu > Cc: > Bcc: > Date: Thu, 9 Jun 2022 10:21:04 -0700 > Subject: [Histonet] Red chroamgen/LeicaBond andBondMax > Can some tell me if the Leica IHC Bond or Bond Max can preform the Red > Chroamgen IHC stain? > John > IMEB > > > > > > ---------- Forwarded message ---------- > From: Tim Morken <tmor...@comcast.net> > To: "John O’Brien" <j...@imebinc.com> > Cc: histonet@lists.utsouthwestern.edu > Bcc: > Date: Thu, 9 Jun 2022 10:58:47 -0700 > Subject: Re: [Histonet] Red chroamgen/LeicaBond andBondMax > John, yes, it is their Bond Refine Red detection kit > > Tim morken > > Sent from my iPhone > > > On Jun 9, 2022, at 10:23 AM, John O’Brien via Histonet < > histonet@lists.utsouthwestern.edu> wrote: > > > > Can some tell me if the Leica IHC Bond or Bond Max can preform the Red > Chroamgen IHC stain? > > John > > IMEB > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > ---------- Forwarded message ---------- > From: "Cartun, Richard" <richard.car...@hhchealth.org> > To: "John O’Brien" <j...@imebinc.com> > Cc: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu > > > Bcc: > Date: Thu, 9 Jun 2022 18:18:55 +0000 > Subject: Re: [Histonet] Red chroamgen/LeicaBond andBondMax > Yes; we run IHC tests everyday on our Bonds with Leica's "Red" detection > kit. You do need to be careful when dehydrating the slides prior to > coverslipping since the chromogenic product is not as stable as DAB. > > Richard > > Richard W. Cartun, MS, PhD > Director, Histology & The Martin M. Berman, MD > Immunopathology/Morphologic Proteomics Laboratory > Assistant Director, Anatomic Pathology > Department of Pathology & Laboratory Medicine > Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 972-1596 Office > (860) 545-2204 (Fax) > > > > -----Original Message----- > From: John O’Brien via Histonet [mailto:histonet@lists.utsouthwestern.edu] > Sent: Thursday, June 9, 2022 1:21 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Red chroamgen/LeicaBond andBondMax > > CAUTION: This email is from outside HHC. USE CARE when opening attachments > or links. > > Can some tell me if the Leica IHC Bond or Bond Max can preform the Red > Chroamgen IHC stain? > John > IMEB > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > > https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!KCs9X-8!dM57DqvKTlhaZFjI21NsFvkIXuDFBoPLUmyLNGPYzxgY5eVBTEyIU-xvMenryn-kwf1k4JZgHYI4QnptD4j7Hj6p6MLk-RL81w$ > > > Reminder: This e-mail and any attachments are subject to the current HHC > email retention policies. Please save or store appropriately in accordance > with policy. > > This e-mail message, including any attachments, is for the sole use of the > intended recipient(s) and may contain confidential and privileged > information. Any unauthorized review, use, disclosure, or distribution is > prohibited. If you are not the intended recipient, or an employee or agent > responsible for delivering the message to the intended recipient, please > contact the sender by reply e-mail and destroy all copies of the original > message, including any attachments. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
