RE: [Histonet] Losing tissue on IHC slides

2013-07-29 Thread Sue Hunter
5:09 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Losing tissue on IHC slides Hi all, I'm looking for some troubleshooting help. In the last couple of months we've been having an increasing trend in losing tissue from our IHC slides. We use a Ventana Benchmark XT. PM

RE: [Histonet] Losing tissue on IHC slides

2013-07-28 Thread Clare Thornton
t-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Deloris Carter [dels...@gmail.com] Sent: Friday, July 26, 2013 5:09 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Losing tissue on IHC slides Hi all, I'm looking for some troubleshooting h

Re: [Histonet] Losing tissue on IHC slides

2013-07-27 Thread Benjamin
So the patient tissue and control are on the same slide, and the control is staying on but patient tissue is falling off? Maybe dont bake the control tissue just let them air dry, the humidity in the slide drying oven might be messing with the super frost plus slides. Its just a coating on the s

Re: [Histonet] Losing tissue on IHC slides

2013-07-26 Thread Hans B Snyder
I agree water is always a potential issue. Tissue can be finicky though. Have you tried poly-L-lysine coated slides? They do help is some occasions. If you need a protocol, let me know, I can write one up, very easy. Hans B Snyder Histologistics 60 Prescott Street Worcester, MA 01605 508-308-7

Re: [Histonet] Losing tissue on IHC slides

2013-07-26 Thread Tony Auge
How are you drying your slides? If in an oven, what's the temp and duration? Try going longer and tap off excess water before drying. Tony Auge HTL (ASCP) QIHC Cell: (651) 373-4768 Email: tony.a...@gmail.com ___ Histonet mailing list Histonet@lists.uts

[Histonet] Losing tissue on IHC slides

2013-07-26 Thread Deloris Carter
Hi all, I'm looking for some troubleshooting help. In the last couple of months we've been having an increasing trend in losing tissue from our IHC slides. We use a Ventana Benchmark XT. PM is done on it quarterly. The problem seems to be with the patient tissue, not the control tissue. We use