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-Original Message-
From: Heckford, Karen - SMMC-SF via Histonet
[mailto:histonet@lists.utsouthwestern.edu]
Sent: Wednesday, 11 May 2016 1:45 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Mela
Cool trick Jeffrey!
On Tue, May 10, 2016 at 12:14 PM, Maxim Peshkov via Histonet <
histonet@lists.utsouthwestern.edu> wrote:
> Karen,
> The bleaching reagents will not compatible before IHC anyway.
> Some tips:
> 1. Do IHC-test as usual and counterstain nuclei with methylene blue
> Loeffler at 3-
Karen,
The bleaching reagents will not compatible before IHC anyway.
Some tips:
1. Do IHC-test as usual and counterstain nuclei with methylene blue Loeffler at
3-5 sec, only for
profile of chromatine. The melanin will stain at green color.
2. Use any red color chromogen for IHC. It will be some co
You are right. Bleaching is a "rough" procedure for the "survival" of sections
and if on top of that you left the section overnight in DiH2O that is a recipe
for disaster, as the one you experienced. Try to do the whole procedure during
the same day.Additionally it seems to me that 6h in potassi
on, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA.
-Original Message-
From: Heckford, Karen - SMMC-SF via Histonet
[mailto:histonet@lists.utsouthwestern.edu]
Sent: Tuesday, May 10, 2016 8:45 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Melanin Bleach
Good Morning,
I
Good Morning,
I need some help. Yesterday I bleached some heavily pigmented tissue. I have
to run some IHC's on them. I bought the bleaching kit from American Master
Tech. I had to put the Permanganate for about 6 hours to get the melanin and
then a couple of minutes in Oxalic Acid. I had
I need to do a melanin bleach with potassium permanganate and oxalic
acid. I do not have any oxalic acid. Does anyone know of a substitute
for the oxalic acid? If I recall correctly, the ox acid gets rid of the
pot permang. If I rinse long enough in water, will that work
Laurie Colbert
