Check www.cqpath.com for more info Verstuurd vanaf mijn iPad
Op 7 dec. 2012 om 19:03 heeft "histonet-requ...@lists.utsouthwestern.edu" <histonet-requ...@lists.utsouthwestern.edu> het volgende geschreven: > Send Histonet mailing list submissions to > histonet@lists.utsouthwestern.edu > > To subscribe or unsubscribe via the World Wide Web, visit > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > or, via email, send a message with subject or body 'help' to > histonet-requ...@lists.utsouthwestern.edu > > You can reach the person managing the list at > histonet-ow...@lists.utsouthwestern.edu > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Histonet digest..." > > > Today's Topics: > > 1. How to open Reichert 820? (Jon Krupp) > 2. DAKO Her2 (Joanne Clark) > 3. Embedding Centers (Tim Wheelock) > 4. RE: Embedding Centers (O'Donnell, Bill) > 5. Paraformaldehyde Solution Recipe for Perfusion (Andrew Coleman) > 6. Re: automated microtomes (Jay Lundgren) > 7. RE: Embedding Centers (Burton, Lynn) > 8. RE: automated microtomes (Rathborne, Toni) > 9. refurbished histology equipment (Patsy Ruegg) > 10. Alcian Blue (Sheila Adey) > 11. RE: Alcian Blue (susan.wal...@hcahealthcare.com) > 12. RE: Alcian Blue (Lynette Pavelich) > 13. Re: Alcian Blue (Rene J Buesa) > 14. Re: Paraformaldehyde Solution Recipe for Perfusion (Geoff) > 15. TISSUE PROCESSOR FOLLOW-UP QUESTION (Tim Wheelock) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Thu, 6 Dec 2012 10:10:31 -0800 > From: Jon Krupp <jkr...@deltacollege.edu> > Subject: [Histonet] How to open Reichert 820? > To: HISTONET <histonet@lists.utsouthwestern.edu> > Message-ID: <09ffdb2c-3cad-4342-b6bb-4a5299d72...@deltacollege.edu> > Content-Type: text/plain; charset=us-ascii > > Hi > > Anyone know how to open the cover of a Reichert 820 microtome? > > This is the model that has a wheel for the coarse advance on the left side. > > I am used to the AO Style, pop the latch & tip it back. These usually have a > crank and a cut out slot on the left. > > This one uses 4 screws from the bottom to secure the lid and there is no cut > out for the coarse advance to slide through. Looks like the wheel has to be > removed to remove the cover. Getting the wheel off is where I am stuck. > > This is an old microtome we have had sitting around, would like to check the > guts and clean it up. > > Jon > > > Jonathan Krupp > Applied Science, Business & Technology > San Joaquin Delta College > 5151 Pacific Ave. > Stockton, CA 95207 > 209-954-5284 > jkr...@deltacollege.edu > > Find us on Facebook @ > Electron Microscopy at SJ Delta College > > > > > > > > > > > > ------------------------------ > > Message: 2 > Date: Thu, 6 Dec 2012 18:32:49 +0000 > From: Joanne Clark <jcl...@pcnm.com> > Subject: [Histonet] DAKO Her2 > To: "histonet@lists.utsouthwestern.edu" > <histonet@lists.utsouthwestern.edu> > Message-ID: > <0494a7d4e8cc254ea2fb81464982e37894637...@s10maild001n4.sh10.lan> > Content-Type: text/plain; charset="us-ascii" > > > Hi All, sorry for how I am submitting this but I have been sending in > questions to the address provided by nothing is going through. > I would like to know who uses DAKO's IVD Her2 IHC marker and how it works. > We want to start running this on our Leica BOND and any info would be much > appreciated. > > Thanks > Joanne Clark, HT(ASCP) > Histology Supervisor > Pathology Consultants of New Mexico > Roswell, NM > > > > ------------------------------ > > Message: 3 > Date: Thu, 06 Dec 2012 15:28:50 -0500 > From: Tim Wheelock <twheel...@mclean.harvard.edu> > Subject: [Histonet] Embedding Centers > To: histonet@lists.utsouthwestern.edu > Message-ID: <50c10002.8070...@mclean.harvard.edu> > Content-Type: text/plain; charset=ISO-8859-1; format=flowed > > Hi Everyone: > > I am also in the market for a new paraffin embedding center. > I have demo-ed or site-visited the Sakura TEK5, the Leica EG1150, and > the Thermo-Fisher HistoStar. > I was wondering if people could give me their critical opinion on these, > or other machines. > What sorts of problems have you had with them. > > I currently have a 25 year old Shandon Embedding Center. I like it a lot. > But I would like to find a machine with a specimen holding tank large > enough to allow me to immerse 300 cassettes all at once. > This is because I infiltrate brain tissue with Tissue Path Paraplast but > embed with Surgipath Embedding Media > So I let the cassettes sit immersed in the Surgipath for an hour or two > before embedding. > > (Until I can buy a new processor, The Shandon's holding tank also serves > as a third processing station, since my Shandon Hypercenter has only 2 > wax reservoirs) > I also do not feel comfortable having the cassettes sitting dry in the > holding tank > > Thanks, > > > Tim Wheelock > Neuropathology Laboratory > Harvard Brain Bank > McLean Hospital > Belmont, MA > > > > > > ------------------------------ > > Message: 4 > Date: Thu, 6 Dec 2012 13:41:28 -0700 > From: "O'Donnell, Bill" <billodonn...@catholichealth.net> > Subject: RE: [Histonet] Embedding Centers > To: "Tim Wheelock" <twheel...@mclean.harvard.edu>, > <histonet@lists.utsouthwestern.edu> > Message-ID: > <4940df6d1c5fdf48931b6966aaef93958ef...@chimsx08.chi.catholichealth.net> > > Content-Type: text/plain; charset="us-ascii" > > Tim - Tissue-Tek 5 has been trouble-free for the 4 years we have had it > with one exception. Early on something went goofy with the dispensing > mechanism. Sakura fixed it promptly and it has been great ever since. - > Bill > > William (Bill) O'Donnell, HT (ASCP) QIHC > Senior Histologist > Good Samaritan Hospital > 10 East 31st Street > Kearney, NE 68847 > > SERENITY is not freedom from the storm, but peace amid the storm. > > Cultivate it in PRAYER! > > > > > > > -----Original Message----- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tim > Wheelock > Sent: Thursday, December 06, 2012 2:29 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Embedding Centers > > Hi Everyone: > > I am also in the market for a new paraffin embedding center. > I have demo-ed or site-visited the Sakura TEK5, the Leica EG1150, and > the Thermo-Fisher HistoStar. > I was wondering if people could give me their critical opinion on these, > or other machines. > What sorts of problems have you had with them. > > I currently have a 25 year old Shandon Embedding Center. I like it a > lot. > But I would like to find a machine with a specimen holding tank large > enough to allow me to immerse 300 cassettes all at once. > This is because I infiltrate brain tissue with Tissue Path Paraplast but > embed with Surgipath Embedding Media So I let the cassettes sit immersed > in the Surgipath for an hour or two before embedding. > > (Until I can buy a new processor, The Shandon's holding tank also serves > as a third processing station, since my Shandon Hypercenter has only 2 > wax reservoirs) I also do not feel comfortable having the cassettes > sitting dry in the holding tank > > Thanks, > > > Tim Wheelock > Neuropathology Laboratory > Harvard Brain Bank > McLean Hospital > Belmont, MA > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > This electronic mail and any attached documents are intended solely for the > named addressee(s) and contain confidential information. If you are not an > addressee, or responsible for delivering this email to an addressee, you have > received this email in error and are notified that reading, copying, or > disclosing this email is prohibited. If you received this email in error, > immediately reply to the sender and delete the message completely from your > computer system. > > > > ------------------------------ > > Message: 5 > Date: Thu, 6 Dec 2012 15:43:09 -0500 > From: Andrew Coleman <andrewcoleman...@gmail.com> > Subject: [Histonet] Paraformaldehyde Solution Recipe for Perfusion > To: histonet@lists.utsouthwestern.edu > Message-ID: > <cafajl_yveruvppytbcblz0mgrnhettcgcbwjgdsxms-_a8w...@mail.gmail.com> > Content-Type: text/plain; charset=ISO-8859-1 > > Hi all, > > We are performing transcardial perfusions in rats using paraformaldehyde in > 0.1M potassium phosphate buffer. > > Can anyone think of any issues that would be caused by using phosphate > buffer made from solely potassium salts (basic and dibasic), rather than a > mixture of sodium and potassium or only the sodium salts? We do our rinse > with 0.1 M PB + Saline and then follow up with solutions just made up in > the potassium phosphate buffer (therefore no Na+). > > Could this cause any tonicity/osmolarity issues? We are trying to > troubleshoot some issues we are having with the perfused tissue. > > Thanks! - Andrew > > > ------------------------------ > > Message: 6 > Date: Thu, 6 Dec 2012 16:21:54 -0500 > From: Jay Lundgren <jaylundg...@gmail.com> > Subject: Re: [Histonet] automated microtomes > To: Lynette Pavelich <lpave...@hurleymc.com> > Cc: "histonet@lists.utsouthwestern.edu" > <histonet@lists.utsouthwestern.edu> > Message-ID: > <cancznuzedkyhvcys4hijtnghmxn_q8syp1nefwpdzwkyzud...@mail.gmail.com> > Content-Type: text/plain; charset=ISO-8859-1 > > Leica is making the sweetest 'tomes out there at the moment, IMHO. > > Sincerely, > > Jay A. Lundgren, M.S., HTL > (ASCP) > > > On Wed, Dec 5, 2012 at 7:10 AM, Lynette Pavelich <lpave...@hurleymc.com>wrote: > >> I am purchasing my second Leica RM2255 automated microtome. Rene' is >> correct in saying that the carpal tunnel syndrome will eventually affect >> every advanced tech by using any microtome manually. On the RM2255, the >> flywheel additionally is automated. You can choose to use the flywheel >> manually or automated by the simultaneous pushing of two buttons. Nice >> feature for us "oldies" to ease us into automation or if you have a tiny >> specimen that you need to take extra care with and want to use manually. >> Companies are very happy to send in a demo to try for a couple weeks. >> >> Happy shopping!! ;) >> Lynette >> >> ________________________________________ >> From: histonet-boun...@lists.utsouthwestern.edu [ >> histonet-boun...@lists.utsouthwestern.edu] on behalf of Rene J Buesa [ >> rjbu...@yahoo.com] >> Sent: Tuesday, December 04, 2012 1:19 PM >> To: Rathborne, Toni; histonet@lists.utsouthwestern.edu >> Subject: Re: [Histonet] automated microtomes >> >> The advantage of the so called automated microtomes (the only thing >> automated about them is the block advance) is that they alleviate wrist >> effort and in some ways prevent carpal tunnel syndrome that affects some >> histotechs (mostly of the "senior persuasion"). >> I would go with the Leica. >> Ren? J. >> >> From: "Rathborne, Toni" <trathbo...@somerset-healthcare.com> >> To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu >> Sent: Tuesday, December 4, 2012 11:48 AM >> Subject: [Histonet] automated microtomes >> >> I'm looking for some opinions about the automated microtomes currently >> available. Which ones do most techs prefer? Which are more reliable? Is >> there an advantage to having a semi-automated microtome? >> Thanks in advance for your replies. >> >> Toni >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ------------------------------ > > Message: 7 > Date: Thu, 6 Dec 2012 21:21:58 +0000 > From: "Burton, Lynn" <lynn.bur...@illinois.gov> > Subject: RE: [Histonet] Embedding Centers > To: Tim Wheelock <twheel...@mclean.harvard.edu>, > "histonet@lists.utsouthwestern.edu" > <histonet@lists.utsouthwestern.edu> > Message-ID: > <ed8bb12ff8b98347be7a3bae9087b546021...@il084exmbx43.illinois.gov> > Content-Type: text/plain; charset="us-ascii" > > Both animal disease labs in the state of Illinois have used the Sakura > machines for the twenty years I have been here with great success. We also > have aSakura processor that has been going for 25 years and a coverslipper > that has only had 3 service calls for minor problems in the past 15+ years. > They make good products. > Lynn Burton > Animal Disease Lab > Galesburg, Il > > -----Original Message----- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tim Wheelock > Sent: Thursday, December 06, 2012 2:29 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Embedding Centers > > Hi Everyone: > > I am also in the market for a new paraffin embedding center. > I have demo-ed or site-visited the Sakura TEK5, the Leica EG1150, and the > Thermo-Fisher HistoStar. > I was wondering if people could give me their critical opinion on these, or > other machines. > What sorts of problems have you had with them. > > I currently have a 25 year old Shandon Embedding Center. I like it a lot. > But I would like to find a machine with a specimen holding tank large enough > to allow me to immerse 300 cassettes all at once. > This is because I infiltrate brain tissue with Tissue Path Paraplast but > embed with Surgipath Embedding Media So I let the cassettes sit immersed in > the Surgipath for an hour or two before embedding. > > (Until I can buy a new processor, The Shandon's holding tank also serves as a > third processing station, since my Shandon Hypercenter has only 2 wax > reservoirs) I also do not feel comfortable having the cassettes sitting dry > in the holding tank > > Thanks, > > > Tim Wheelock > Neuropathology Laboratory > Harvard Brain Bank > McLean Hospital > Belmont, MA > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 8 > Date: Thu, 6 Dec 2012 21:47:18 +0000 > From: "Rathborne, Toni" <trathbo...@somerset-healthcare.com> > Subject: RE: [Histonet] automated microtomes > To: "'Jay Lundgren'" <jaylundg...@gmail.com>, Lynette Pavelich > <lpave...@hurleymc.com> > Cc: "histonet@lists.utsouthwestern.edu" > <histonet@lists.utsouthwestern.edu> > Message-ID: > > <3ad061fe740d464fac7bf6b5cfb757071f1f6...@smcmail02.somerset-healthcare.com> > > Content-Type: text/plain; charset="iso-8859-1" > > Thanks all for your valued opinions. I have contacted Leica, and am now > waiting for a demo! > > From: Jay Lundgren [mailto:jaylundg...@gmail.com] > Sent: Thursday, December 06, 2012 4:22 PM > To: Lynette Pavelich > Cc: Rene J Buesa; Rathborne, Toni; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] automated microtomes > > Leica is making the sweetest 'tomes out there at the moment, IMHO. > > Sincerely, > > Jay A. Lundgren, M.S., HTL > (ASCP) > > On Wed, Dec 5, 2012 at 7:10 AM, Lynette Pavelich > <lpave...@hurleymc.com<mailto:lpave...@hurleymc.com>> wrote: > I am purchasing my second Leica RM2255 automated microtome. Rene' is correct > in saying that the carpal tunnel syndrome will eventually affect every > advanced tech by using any microtome manually. On the RM2255, the flywheel > additionally is automated. You can choose to use the flywheel manually or > automated by the simultaneous pushing of two buttons. Nice feature for us > "oldies" to ease us into automation or if you have a tiny specimen that you > need to take extra care with and want to use manually. > Companies are very happy to send in a demo to try for a couple weeks. > > Happy shopping!! ;) > Lynette > > ________________________________________ > From: > histonet-boun...@lists.utsouthwestern.edu<mailto:histonet-boun...@lists.utsouthwestern.edu> > > [histonet-boun...@lists.utsouthwestern.edu<mailto:histonet-boun...@lists.utsouthwestern.edu>] > on behalf of Rene J Buesa [rjbu...@yahoo.com<mailto:rjbu...@yahoo.com>] > Sent: Tuesday, December 04, 2012 1:19 PM > To: Rathborne, Toni; > histonet@lists.utsouthwestern.edu<mailto:histonet@lists.utsouthwestern.edu> > Subject: Re: [Histonet] automated microtomes > > The advantage of the so called automated microtomes (the only thing automated > about them is the block advance) is that they alleviate wrist effort and in > some ways prevent carpal tunnel syndrome that affects some histotechs (mostly > of the "senior persuasion"). > I would go with the Leica. > Ren? J. > > From: "Rathborne, Toni" > <trathbo...@somerset-healthcare.com<mailto:trathbo...@somerset-healthcare.com>> > To: > "histonet@lists.utsouthwestern.edu<mailto:histonet@lists.utsouthwestern.edu>" > <histonet@lists.utsouthwestern.edu<mailto:histonet@lists.utsouthwestern.edu>> > Sent: Tuesday, December 4, 2012 11:48 AM > Subject: [Histonet] automated microtomes > > I'm looking for some opinions about the automated microtomes currently > available. Which ones do most techs prefer? Which are more reliable? Is there > an advantage to having a semi-automated microtome? > Thanks in advance for your replies. > > Toni > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu<mailto:Histonet@lists.utsouthwestern.edu> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu<mailto:Histonet@lists.utsouthwestern.edu> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu<mailto:Histonet@lists.utsouthwestern.edu> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 9 > Date: Thu, 6 Dec 2012 15:40:47 -0700 > From: "Patsy Ruegg" <pru...@ihctech.net> > Subject: [Histonet] refurbished histology equipment > To: <histonet@lists.utsouthwestern.edu> > Message-ID: <311FA59E257C47B99E7C538F1DB384AE@DESKTOP3> > Content-Type: text/plain; charset="us-ascii" > > Does anyone have a favorite vendor they would recommend to me? I am mostly > looking for cryostats right now, but interested in other things, tissue > processors, IHC stainers, etc. > > > > Patsy Ruegg, HT(ASCP)QIHC > > Ruegg IHC Consulting, LLC > > 40864 Arkansas Ave > > Bennett, CO 80102 > > Phone: 303-644-4538 > > Fax: 720-859-4110 > > <mailto:pru...@ihctech.net> pru...@ihctech.net > > > > > > ------------------------------ > > Message: 10 > Date: Thu, 6 Dec 2012 20:58:10 -0500 > From: Sheila Adey <sa...@hotmail.ca> > Subject: [Histonet] Alcian Blue > To: "histonet@lists.utsouthwestern.edu" > <histonet@lists.utsouthwestern.edu> > Message-ID: <bay154-w12f429f13129f56a6b76c5c6...@phx.gbl> > Content-Type: text/plain; charset="iso-8859-1" > > > Hi Everyone: > Can anyone tell me why some Alcian blue procedures say 30 min in Alcian Blue > and some say 10 min?Our control works well at 10 minutes but today I had a > Dr. say that he expected a small amount of cells to stain and they didn't in > an esophagus bx.So, now I'm wondering if b/c most of the procedures that I've > read say 30 min, that's what would be best? > Thanks > Sheila > > ------------------------------ > > Message: 11 > Date: Fri, 7 Dec 2012 02:04:37 -0600 > From: <susan.wal...@hcahealthcare.com> > Subject: RE: [Histonet] Alcian Blue > To: <sa...@hotmail.ca>, <histonet@lists.utsouthwestern.edu> > Message-ID: > <4bf03f5404ebde409af9232da74b9ded2df8521...@fwdcwpmsgcms09.hca.corpad.net> > > Content-Type: text/plain; charset="us-ascii" > > We've always used 30 minutes and it has never failed. > > -----Original Message----- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sheila Adey > Sent: Thursday, December 06, 2012 8:58 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Alcian Blue > > > Hi Everyone: > Can anyone tell me why some Alcian blue procedures say 30 min in Alcian Blue > and some say 10 min?Our control works well at 10 minutes but today I had a > Dr. say that he expected a small amount of cells to stain and they didn't in > an esophagus bx.So, now I'm wondering if b/c most of the procedures that I've > read say 30 min, that's what would be best? > Thanks > Sheila _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > ------------------------------ > > Message: 12 > Date: Fri, 7 Dec 2012 12:06:15 +0000 > From: Lynette Pavelich <lpave...@hurleymc.com> > Subject: RE: [Histonet] Alcian Blue > To: "susan.wal...@hcahealthcare.com" <susan.wal...@hcahealthcare.com>, > "sa...@hotmail.ca" <sa...@hotmail.ca>, > "histonet@lists.utsouthwestern.edu" > <histonet@lists.utsouthwestern.edu> > Message-ID: > <89f4666a496dc949a819ecc40e11c867bf56c...@exchangemb1.hmc.hurleymc.com> > > Content-Type: text/plain; charset="us-ascii" > > 30 here..... > > Lynette > > ________________________________________ > From: histonet-boun...@lists.utsouthwestern.edu > [histonet-boun...@lists.utsouthwestern.edu] on behalf of > susan.wal...@hcahealthcare.com [susan.wal...@hcahealthcare.com] > Sent: Friday, December 07, 2012 3:04 AM > To: sa...@hotmail.ca; histonet@lists.utsouthwestern.edu > Subject: RE: [Histonet] Alcian Blue > > We've always used 30 minutes and it has never failed. > > -----Original Message----- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sheila Adey > Sent: Thursday, December 06, 2012 8:58 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Alcian Blue > > > Hi Everyone: > Can anyone tell me why some Alcian blue procedures say 30 min in Alcian Blue > and some say 10 min?Our control works well at 10 minutes but today I had a > Dr. say that he expected a small amount of cells to stain and they didn't in > an esophagus bx.So, now I'm wondering if b/c most of the procedures that I've > read say 30 min, that's what would be best? > Thanks > Sheila > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > ------------------------------ > > Message: 13 > Date: Fri, 7 Dec 2012 06:11:50 -0800 (PST) > From: Rene J Buesa <rjbu...@yahoo.com> > Subject: Re: [Histonet] Alcian Blue > To: Sheila Adey <sa...@hotmail.ca>, > "histonet@lists.utsouthwestern.edu" > <histonet@lists.utsouthwestern.edu> > Message-ID: > <1354889510.81416.yahoomail...@web163105.mail.bf1.yahoo.com> > Content-Type: text/plain; charset=iso-8859-1 > > If after many successful runs with your Alcian Blue reagents you get a > complaint about either strength of staining or cells not staining at all, > most likely you have a problem with the solutions. If you prepare them "in > house" check the preparation date and prepare a fresh solution. The general > procedure calls for 30 min, unless you use a microwave oven version of the > staining protocol. > Ren? J. > > From: Sheila Adey <sa...@hotmail.ca> > To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> > Sent: Thursday, December 6, 2012 8:58 PM > Subject: [Histonet] Alcian Blue > > > Hi Everyone: > Can anyone tell me why some Alcian blue procedures say 30 min in Alcian Blue > and some say 10 min?Our control works well at 10 minutes but today I had a > Dr. say that he expected a small amount of cells to stain and they didn't in > an esophagus bx.So, now I'm wondering if b/c most of the procedures that I've > read say 30 min, that's what would be best? > Thanks > Sheila ??? ??? ??? ? ??? ??? ? _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ > > Message: 14 > Date: Fri, 07 Dec 2012 10:50:09 -0500 > From: Geoff <mcaul...@umdnj.edu> > Subject: Re: [Histonet] Paraformaldehyde Solution Recipe for Perfusion > To: histonet@lists.utsouthwestern.edu > Message-ID: <50c21031.7060...@umdnj.edu> > Content-Type: text/plain; CHARSET=US-ASCII; format=flowed > > What issues are you having? That might help the list diagnose the problem. > > Geoff > > On 12/6/2012 3:43 PM, Andrew Coleman wrote: >> Hi all, >> >> We are performing transcardial perfusions in rats using paraformaldehyde in >> 0.1M potassium phosphate buffer. >> >> Can anyone think of any issues that would be caused by using phosphate >> buffer made from solely potassium salts (basic and dibasic), rather than a >> mixture of sodium and potassium or only the sodium salts? We do our rinse >> with 0.1 M PB + Saline and then follow up with solutions just made up in >> the potassium phosphate buffer (therefore no Na+). >> >> Could this cause any tonicity/osmolarity issues? We are trying to >> troubleshoot some issues we are having with the perfused tissue. >> >> Thanks! - Andrew >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- > -- > ********************************************** > Geoff McAuliffe, Ph.D. > Neuroscience and Cell Biology > Robert Wood Johnson Medical School > 675 Hoes Lane, Piscataway, NJ 08854 > voice: (732)-235-4583; fax: -4029 > mcaul...@umdnj.edu > ********************************************** > > > > > > ------------------------------ > > Message: 15 > Date: Fri, 07 Dec 2012 12:44:11 -0500 > From: Tim Wheelock <twheel...@mclean.harvard.edu> > Subject: [Histonet] TISSUE PROCESSOR FOLLOW-UP QUESTION > To: Histonet@lists.utsouthwestern.edu > Message-ID: <50c22aeb.6070...@mclean.harvard.edu> > Content-Type: text/plain; charset=ISO-8859-1; format=flowed > > Hi All: > > First, thank you for all your feedback on the processors. > > I site-visited a VIP6 at a local hospital > From my understanding the VIP6 can rotate absolute alcohol and xylene > (using the bulk reservoirs), as well as the paraffin stations, but it > cannot rotate other concentrations of alcohol based on hydrometer readings. > Am I correct in this appraisal? > > Tim Wheelock > Neuropathology Laboratory > Harvard Brain Bank > McLean Hospital > Belmont, MA > > > > ------------------------------ > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > End of Histonet Digest, Vol 109, Issue 9 > **************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
