What we do here is run a mix of expected negative and positive cases this way
we are sure we are not getting exogenous staining. Depending on the antibody
we try to run at least a mix of 25 cases. In some cases the antibody proves
difficult to get a large amount of positive cases and we make
We are beginning the process of validating new antibodies using the BenchMark
XT but have some questions for validation.
Question: For a positive reagent control that stains appropriately positive,
is a known control block used? If not, what is used?
Question: For a negative reagent control th
Dear All,
We are looking negative control for FLI-1, as positive control we are using
(PNET) on paraffin section successfully.
Our pathologist gave us Wilm's tumor and IDC for Negative control but it not
work good.
Thanks
Muhammad Tahseen
Sr. Supervisor Histology
SKMCH&RC Lahore
Pakistan
_
At the first of August Richard Cartun posted that CAP had a new negative
control policy. Does anyone know if it is already in effect and if it
would apply to up coming inspections.
Judith Pardue
Histology Supervisor
Memorial Hospital
Chattanooga, Tn.
judith_par...@memorial.org
This elec
Hello,
Does anyone know of a good control tissue absent of (or with few) macrophages?
I have seen a number of postings regarding tissue positive for various
inflammatory markers (F4/80, MOMA. CD68).
I am running F4/80 (Serotec, Rat anti-ms F4/80) on ms. tissue. I have a number
of positive
We have not done negative controls, but, have recently started placing both a
negative and positive control on slides for Congo Red based on recommendation
by Mayo. We are using a keloid scar. They state that on consult cases, they
have often noticed overstaining on high collagen cases. Our pa
