lem via Histonet"
wrote:
Could it be due to incomplete deparaffinization?
Willem
Van: Vickroy, James via Histonet [histonet@lists.utsouthwestern.edu]
Verzonden: dinsdag 16 februari 2016 18:10
Aan: histonet@lists.utsouthwestern.edu
Onderwer
From: Hoekert, Willem via Histonet [histonet@lists.utsouthwestern.edu]
Sent: Wednesday, February 17, 2016 11:54 PM
To: Vickroy, James; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Nuclear Bubbling
Could it be due to incomplete deparaffinization?
Willem
Could it be due to incomplete deparaffinization?
Willem
Van: Vickroy, James via Histonet [histonet@lists.utsouthwestern.edu]
Verzonden: dinsdag 16 februari 2016 18:10
Aan: histonet@lists.utsouthwestern.edu
Onderwerp: [Histonet] Nuclear Bubbling
m or saving them to any media storage device.
--
Message: 2
Date: Tue, 16 Feb 2016 18:12:27 + (UTC)
From: Rene J Buesa
To: "Vickroy, James" ,
"histonet@lists.utsouthwestern.edu"
Subject: Re: [Histonet] Nuclear Bubbling
Message-ID
Jim,
Are you drying the slides in an oven before the heat stage on the Prisma? If
you use an oven, what is the temperature setting and for how long are slides
kept in? Carson and Hladik recommends 60 degrees for less than 1 hour.
You mentioned, recycled formalin, what is the pH of the formalin?
.com
> ]
> Sent: Tuesday, February 16, 2016 1:56 PM
> To: Manfre, Philip; Rene J Buesa; Vickroy, James
> Cc: جمال الرويحي; histonet@lists.utsouthwestern.edu
> Subject: Re: [Histonet] Nuclear Bubbling
>
> Great, I agree
>
>
>
> Regards
>
> Jamal R
ickroy, James
Cc: جمال الرويحي; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Nuclear Bubbling
Great, I agree
Regards
Jamal Rowaihi
Anatomic Pathology Supervisor
Al Borg Medical Laboratories
Sent from my cell phone
Original message
From: "Manfre, Philip v
Dear James:
Two chained events take place on your issue:
1-sections not entirely drained,water remains even minimally under
sections;
2- you put these sections in the oven, the heat literally "explodes" the
nuclear bubbles and creates a hole with no cromatin to stain.
My kind regards,
Carlos D
quot;
Cc: histonet@lists.utsouthwestern.edu Subject:
Re: [Histonet] Nuclear Bubbling
Sort of a rude response to someone looking for help.
-Original Message-
From: Rene J Buesa via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Tuesday, February 16, 2016 1:12 PM
To: Vick
Sort of a rude response to someone looking for help.
-Original Message-
From: Rene J Buesa via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Tuesday, February 16, 2016 1:12 PM
To: Vickroy, James; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Nuclear Bubbling
If I
"histonet@lists.utsouthwestern.edu"
Date: 02/16/2016 10:13 AM
Subject: Re: [Histonet] Nuclear Bubbling
If I remember correctly, this issue has been discussed previously.The
general consensus as to the cause of nuclear "bubbling" (in reality a lack
of staining in the nuclear a
If I remember correctly, this issue has been discussed previously.The general
consensus as to the cause of nuclear "bubbling" (in reality a lack of staining
in the nuclear area) has been attributed to an incomplete section drying.After
the section has be "fished" from the water bath, if the slid
Struggling to find an answer. We do a lot of GI biopsies in our lab.
Sometimes they look wonderful without any nuclear bubbling, other times the
bubbling is pretty intense. Since nuclear bubbling is often attributed to
incomplete fixation we of course have investigated the fixation times.
udrun Lang
-Ursprüngliche Nachricht-
Von: Teri Johnson via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Gesendet: Freitag, 29. Jänner 2016 19:49
An: 'histonet@lists.utsouthwestern.edu'
Betreff: Re: [Histonet] Nuclear bubbling artifact
Hi James,
Nuclear bubbling artifact i
Hi James,
Nuclear bubbling artifact is most commonly seen in formalin fixed epithelial
cells, and GI biopsies are among those samples that are particularly
susceptible to it. It has been linked to inadequate fixation and also to
heating of slides prior to staining without complete air-drying of
"Nuclear bubbling", manifested as round unstained areas in the nucleus, is
caused by incomplete dehydration of the section before staining. There is a
review on the subject that I cannot find at this moment.René
On Friday, January 29, 2016 10:42 AM, "Vickroy, James via Histonet"
wrote:
Traditionally we regard nuclear bubbling as incomplete fixation however I'm not
so sure that nuclear bubbling can't be caused by additional processing
problems. This morning I have some GI biopsies that fixed for nearly 18 hrs
that have a large amount of nuclear bubbling. We run the biopsies
ursday, May 12, 2011 1:41 PM
To: Johnson, Nacaela; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] nuclear bubbling
Nacaela:
Heat by itself is not the cause, but the heat applied to sections that have
not been properly drained and still have water left between them and the
slide, is.
You hav
Buesa; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] nuclear bubbling
Another individual called me and commented further, which clarified your
statement. It is actually poor lab practice to combine the drying/baking.
These steps should really be done separately so as to not "trap
; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] nuclear bubbling
So there really isn't a point in using an oven to dry the slides? I currently
dry my slides for an hour at 55-60 degrees C.
Thanks,
Nacaela Johnson, B.S. HTL (ASCP)CM
Histotechnologist
KCCC Pathology
12000 110th St., St
t: Re: [Histonet] nuclear bubbling
Nacaela:
Heat by itself is not the cause, but the heat applied to sections that have not
been properly drained and still have water left between them and the slide, is.
You have to drain the sections properly before heating them.
Using formalin is not the cause.
R
caela"
To: histonet@lists.utsouthwestern.edu
Sent: Thursday, May 12, 2011 12:51 PM
Subject: [Histonet] nuclear bubbling
Has anyone one had problems with nuclear bubbling? I have read about
two different causes. (1) Formalin itself is known to cause the issue
and (2) heating the tissue at a
Johnson,
Nacaela
Gesendet: Donnerstag, 12. Mai 2011 18:51
An: histonet@lists.utsouthwestern.edu
Betreff: [Histonet] nuclear bubbling
Has anyone one had problems with nuclear bubbling? I have read about
two different causes. (1) Formalin itself is known to cause the issue
and (2) heating the tissue at
Has anyone one had problems with nuclear bubbling? I have read about
two different causes. (1) Formalin itself is known to cause the issue
and (2) heating the tissue at a high temp (70 degrees C or above) while
drying. The latter is definitely not happening, but I do use formalin.
I am in the pr
Peshkov,
Russia,
Taganrog.
--- On Fri, 7/17/09, Harrison, Sandra C. wrote:
From: Harrison, Sandra C.
Subject: RE: [Histonet] nuclear bubbling
To: "Histonet"
Date: Friday, July 17, 2009, 5:27 PM
How about microwaving to dry slides?
Can that cause nuclear bubbling?
When you say complete
: [Histonet] nuclear bubbling
To: "Histonet"
Date: Friday, July 17, 2009, 5:27 PM
How about microwaving to dry slides? Can that cause nuclear bubbling?
When you say completely drained off, does that mean your slides have to be
completely dry prior to placing them in the oven?
Tha
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Wednesday, July 15, 2009 2:59 PM
To: Histonet; Joyce Cline
Subject: Re: [Histonet] nuclear bubbling
Anybody can experience nuclear bubbling in any type tissue as long as the
sections as set to dry at high temperature
Sub optimal fixation and as Rene said; drying off at high temperatures.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joyce
Cline
Sent: 15 July 2009 20:45
To: Histonet
Subject: [Histonet] nuclear
Anybody can experience nuclear bubbling in any type tissue as long as the
sections as set to dry at high temperature BEFORE they are completely drained
off!
René J.
--- On Wed, 7/15/09, Joyce Cline wrote:
From: Joyce Cline
Subject: [Histonet] nuclear bubbling
To: "Histonet"
Date:
Has anyone experienced nuclear bubbling on prostate biopsies?
Joyce
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