Few more things:
1. The mixed/working solution is good for 1-2 hours only. If you
filter it for a long time, this might use the good working time and then you
have trouble, because it just won't work well for you. I just poor the stain
and the water in a glass cylinder and shake it. That'
Hi Akemi,
Order the stain from Electron Microscopy Sciences - cat # 26503-02. It
comes as 250 ml solution and is a lot less expensive than some other
sources. I guess you can probably get it through VWR.
Mix 3 parts of this stock with 2 parts dist. Water and let it stand for 10
min.
Filt
>
> -Ursprüngliche Nachricht-
> Von: histonet-boun...@lists.utsouthwestern.edu
> [mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Akemi
> Allison
> Gesendet: Mittwoch, 26. Jänner 2011 18:16
> An: histonet
> Betreff: [Histonet] Oil Red O for FS on Muscle
>
-boun...@lists.utsouthwestern.edu] Im Auftrag von Akemi
Allison
Gesendet: Mittwoch, 26. Jänner 2011 18:16
An: histonet
Betreff: [Histonet] Oil Red O for FS on Muscle
Happy hump day!
Does anyone have a good procedure for Oil Red O for FS on Muscle.
The procedure the lab I am working with is having a great
I've done many of these stains and I get my ORO stain from Poly Scientific.
Actually I use the whole kit and I use Freida's protocol in the second edition.
That includes fixing in 37% formaldehyde. I filter the ORO just before use and
usually get nice clean slides. If there is a residue it isn't
:
1567 Skyway Drive, Unit E
Longmont, Colorado 80504
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Akemi Allison
Sent: Wednesday, January 26, 2011 10:16 AM
To: histonet
Subject: [Histonet] Oil Red O for FS on
f Of Akemi Allison
Sent: Wednesday, January 26, 2011 11:16 AM
To: histonet
Subject: [Histonet] Oil Red O for FS on Muscle
Happy hump day!
Does anyone have a good procedure for Oil Red O for FS on Muscle.
The procedure the lab I am working with is having a great deal of
problems with their mus
Happy hump day!
Does anyone have a good procedure for Oil Red O for FS on Muscle.
The procedure the lab I am working with is having a great deal of
problems with their muscle biopsy panel. I am trouble-shooting some
of the issues for the 1-Step Trichrome 3.4 pH, NADH, AT Pase, I am
wo
