u, October 14, 2010 8:08:17 AM
Subject: RE: [Histonet] Optimal processing for prostate needle biopsies
Phyllis,
We hold all our needle cores overnight and pre-process for 15 min. The problems
we are having is shrunken nuclei and nucleoli are not distinct even in
carcinoma
cases. Cracking ar
but staining with
Mayer's brings out nucleoli better.
From: Phyllis Thaxton [dch...@yahoo.com]
Sent: Wednesday, October 13, 2010 10:37 AM
To: Gupta, Nilesh; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Optimal processing for prostate needle bio
good.
Phyllis Thaxton HT(ASCP)QIHC
DCH Regional Medical Center
Tuscaloosa, AL
From: "Gupta, Nilesh"
To: "histonet@lists.utsouthwestern.edu"
Sent: Tue, October 12, 2010 12:02:23 PM
Subject: [Histonet] Optimal processing for prostate needl
ause it is regressive and can be
controlled better than Harris that is progressive and will need more staining
time as it gets older/weaker
René J.
--- On Tue, 10/12/10, Gupta, Nilesh wrote:
From: Gupta, Nilesh
Subject: [Histonet] Optimal processing for prostate needle biopsies
To:
I have a few questions regrading processing of prostate needle biopsies.
1. What is optimal fixation time that the needle cores should be fixed for
before loading these on the processors.
2. Our cores are processed on Tissue tek Xpress x120 but the morphology is not
so good. I'd like to know if