Re: [Histonet] Optimal processing for prostate needle biopsies

2010-10-15 Thread Phyllis Thaxton
u, October 14, 2010 8:08:17 AM Subject: RE: [Histonet] Optimal processing for prostate needle biopsies Phyllis, We hold all our needle cores overnight and pre-process for 15 min. The problems we are having is shrunken nuclei and nucleoli are not distinct even in carcinoma cases. Cracking ar

RE: [Histonet] Optimal processing for prostate needle biopsies

2010-10-14 Thread Gupta, Nilesh
but staining with Mayer's brings out nucleoli better. From: Phyllis Thaxton [dch...@yahoo.com] Sent: Wednesday, October 13, 2010 10:37 AM To: Gupta, Nilesh; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Optimal processing for prostate needle bio

Re: [Histonet] Optimal processing for prostate needle biopsies

2010-10-13 Thread Phyllis Thaxton
good.  Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL From: "Gupta, Nilesh" To: "histonet@lists.utsouthwestern.edu" Sent: Tue, October 12, 2010 12:02:23 PM Subject: [Histonet] Optimal processing for prostate needl

Re: [Histonet] Optimal processing for prostate needle biopsies

2010-10-12 Thread Rene J Buesa
ause it is regressive and can be controlled better than Harris that is progressive and will need more staining time as it gets older/weaker René J. --- On Tue, 10/12/10, Gupta, Nilesh wrote: From: Gupta, Nilesh Subject: [Histonet] Optimal processing for prostate needle biopsies To:

[Histonet] Optimal processing for prostate needle biopsies

2010-10-12 Thread Gupta, Nilesh
I have a few questions regrading processing of prostate needle biopsies. 1. What is optimal fixation time that the needle cores should be fixed for before loading these on the processors. 2. Our cores are processed on Tissue tek Xpress x120 but the morphology is not so good. I'd like to know if