Laurie and Pablo, doing a soft antigen retrieval in a citrate buffer
solution pH6 for 10 minutes after hydrating your slides
will allow you to improve the quality of nuclear stain.
El 29/04/2015 a las 13:07, pablo.sanc...@usc.es escribió:
As I usually process gross pieces of bone -that need abou
: Wednesday, April 29, 2015 12:21 PM
To: pablo.sanc...@usc.es
Cc: Histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Over- decalcified tissue
I would suggest not allowing decalcification to be extended. Better to leave in
fixative until you can control the time of endpoint of decalcification
I would suggest not allowing decalcification to be extended. Better to leave in
fixative until you can control the time of endpoint of decalcification. What
type of decal solution are you using? I am not a big fan of trying to adjust
the chemistry of the stain to compensate for over decalcificat
As I usually process gross pieces of bone -that need abouth thirty
hours decalcifying- I suffer the same nuissance. Also would thank any
hint.
Pablo Sanchez
Laurie Colbert escribiu:
I have tissue that was left in decal over the weekend and now has
very poor nuclear staining. Is there
I have tissue that was left in decal over the weekend and now has very poor
nuclear staining. Is there a "fix" for this so that I can get better nuclear
staining (other than restaining for a long time in the hematoxylin)?
Laurie Colbert, HT (ASCP)
Histology Supervisor
PATH MD
8158 Beverly Blvd.
