We use a .5% to 1% solution of diastase for our fungal pas (so the docs can see
the fungus better) and leave it in for 25-30 min at room temp and have had
great results. We use American mastertech brand of diastase.
Andy Fortune
This message and accompanying documents are covered by the Electro
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Subject: Re: [Histonet] PAS-diastase staining
Hi,
Sigma has discontinued selling type VIII-alfa amylase from barley
(catalogue ref A2771). Anyone knows any other commercial source of
this enzyme or if the other amylase (other types of alfa amylase or
beta amylase) work for PAS-D
, May 29, 2015 6:35 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] PAS-diastase staining
Hi there,
I am trying to do a PAA-diastase in formalin-fixed embedded samples
(ovine liver). I would be most grateful if someone could give me a
hand in:
-Protocol (enzyme concentration
esa; Julio Benavides; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] PAS-diastase staining
I have been using the spit method for years with great results. Depar slide to
water, add saliva directly to slide for 10 minutes, rinse and stain PAS as
usual. Also American MasterTech has diastase
Hi there,
thank you all very much for your comments and protocols on the
PAS-diastase. Although funny thing that cheaper option, I think I stick
to the Sigma enzyme first.
Update on the formalin "crystals". It is actually, as you suggested,
white granular precipitate. There was so much of it
Sigma porcine diastase is very good.A cheaper option is very disgusting but had
been done for decades, just spit on the section but that will carry bacteria
and, although I have seen doing it, I have never done it.René J.
On Friday, May 29, 2015 10:01 AM, Julio Benavides
wrote:
H
...@yahoo.com]
Sent: Friday, May 29, 2015 11:17 AM
To: Julio Benavides; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] PAS-diastase staining
Sigma porcine diastase is very good.A cheaper option is very disgusting but had
been done for decades, just spit on the section but that will carry
Hi there,
I am trying to do a PAA-diastase in formalin-fixed embedded samples
(ovine liver). I would be most grateful if someone could give me a hand in:
-Protocol (enzyme concentration, time of digestion...) for diastase
digestion (I am assuming that PAS afterwards is the normal protocol for
Tony Henwood (SCHN) would like to recall the message, "[Histonet] PAS Diastase".
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-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Geoff McAuliffe
Sent: Friday, 16 March 2012 12:55 AM
To: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] PAS Di
Jennifer,
No you are not.
Matt Lunetta
BS HT(ASCP)
Longmont United Hospital
Message: 9
Date: Wed, 14 Mar 2012 22:47:17 -0700
From: Jennifer MacDonald
Subject: [Histonet] PAS Diastase
To: histonet@lists.utsouthwestern.edu
Message-ID:
Content-Type: text/plain; charset="US-ASCII"
Hi
I agree with Rene. A control (undigested) slide is necessary.
Geoff
On 3/15/2012 1:47 AM, Jennifer MacDonald wrote:
Hi All,
At a local lab when a pathologist orders a PAS diastase the
histotechnicians do just one slide with diastase. They do not do an
undigested slide. How would the pathologi
No, you are correct. The tech should always give the pathologists one with and
another without diastase.
René J.
--- On Thu, 3/15/12, Jennifer MacDonald wrote:
From: Jennifer MacDonald
Subject: [Histonet] PAS Diastase
To: histonet@lists.utsouthwestern.edu
Date: Thursday, March 15, 2012, 1:47
Hi All,
At a local lab when a pathologist orders a PAS diastase the
histotechnicians do just one slide with diastase. They do not do an
undigested slide. How would the pathologist know if the digested slide
had a glycogen to begin with? Am I over thinking this?
Thanks,
Jennifer
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