Sorry, I should have addressed this to Erin.
On Tue, Mar 15, 2011 at 12:12 PM, Jay Lundgren wrote:
> Geoff,
>
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Geoff,
A PAS is not really the best stain for basement membranes. I think
your Pathologist wants to look at a PAMS. You can make the basement
membrane look like you drew it on the slide with a Sharpie marker. After
the silver you can counterstain however you want.
Sincerely,
Jay A. Lund
Make the periodic acid fresh that day.
Use 3 2 minute washes in 0.5% sodium meta-bisulfite instead of water.
Geoff
On 3/15/2011 10:16 AM, Martin, Erin wrote:
Hi all,
I'm having a problem with my PAS stain. My pathologist says that glycogen and
fungus stain beautifully but basement membranes
tonet] PAS for basement membrane
Hi all,
I'm having a problem with my PAS stain. My pathologist says that glycogen
and fungus stain beautifully but basement membranes are pale. We are
using 0.5% periodic acid for 10 minutes and schiff's for 25 minutes
followed by 5 min rin
Hi all,
I'm having a problem with my PAS stain. My pathologist says that glycogen and
fungus stain beautifully but basement membranes are pale. We are using 0.5%
periodic acid for 10 minutes and schiff's for 25 minutes followed by 5 min
rinse in warm/hot water. Any thoughts? Thank you in ad
