Hi Charles
Getting three shades of pink depends a kit on your dehydration steps after
eosin. Eosin is more soluble in water than alcohol so you have to watch how
much water is being absorbed from the atmosphere and change/rotate alcohols
when you see the last absolute turning pink, also if yo
Heat can cause poor chromatin staining. Is the two shades of eosin only
on the biopsies, or all tissues?
From: Charles Riley via Histonet
To: histonet@lists.utsouthwestern.edu
Date: 06/16/2016 11:19 AM
Subject:[Histonet] Poor Staining
Can over processing small biopsies
Can over processing small biopsies lead to poor chromatin staining? Also
can this cause an issue with the eosin staining ( for example only getting
to shades of pink instead of three)?
Please give me any feedback you can
--
Charles Riley HT(ASCP)CM
Histopathology Coordinator/ Mohs
