Formalin fixes slowly, even more slowly at 4 degrees C. Even though the
fix may penetrate, fixation will not be complete.
I suggest a formalin+alcohol+acetic acid fix at room temp for 24 hours.
There are many, many variations of this fix. Lillie recommends 85 ml of
95% EtOH, 10 ml conc. formalin
: SPAM-LOW: [Histonet] Problem with liver fixation
Dear All,
I'm trying to use liver sections in paraffin blocks for IHC, but get no
signal at all, doesn't matter what antibody i'm using.
I'm using section about 3mm thick (the natural thickness of mouse liver) and
about 5-7mm W
Bouin's will not do better than what you describe. Are you using antigen
retrieval?
René J.
--- On Sun, 9/5/10, Itai Moshe wrote:
From: Itai Moshe
Subject: [Histonet] Problem with liver fixation
To: histonet@lists.utsouthwestern.edu
Date: Sunday, September 5, 2010, 8:37 AM
Dear All
Dear All,
I'm trying to use liver sections in paraffin blocks for IHC, but get no
signal at all, doesn't matter what antibody i'm using.
I'm using section about 3mm thick (the natural thickness of mouse liver) and
about 5-7mm Width and Length.
My fixation protocol is like this:
1) immediately aft
