Re: [Histonet] Processing lavages into cell pellets...

2010-03-15 Thread Anthony Reilly
Hi Jennifer I realise there are products like Histogel about of which I have no knowledge however I have been using agar for over 30 years because I can get it easily and gratis from our microbiology department. My experience is to spin down the cells, remove the supernatent, then add fixati

[Histonet] Processing lavages into cell pellets...

2010-03-15 Thread Johnson, Jennifer(Hist)
Hi, I am looking for a little advice from anyone who has experience processing lavage fluid into cell pellets that can be sectioned and stained. I need to work out a protocol using lavages from knockout mice that may eventually be used to look at human patients. In both models of disease I expec