uthwestern.edu;
> histonet-requ...@lists.utsouthwestern.edu
> CC:
> Subject: [Histonet] Quenching auto fluorescence in human tissue
>
> Hi all,
>
>
>
> I have been experiencing trouble with my secondary antibody when doing
> my immuno with human colon tissue.
>
> I use to use FITC 48
Hi all,
I have been experiencing trouble with my secondary antibody when doing
my immuno with human colon tissue.
I use to use FITC 488 as my secondary antibody and changed over to
Alexa488 due to the FITC's manufacturing stop.
The auto fluorescence of the human tissue is quenched with Chicag
-activity is restored.
Gudrun Lang
-Ursprüngliche Nachricht-
Von: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Candice
Smoots
Gesendet: Mittwoch, 29. Juni 2011 17:21
An: Histonet
Betreff: [Histonet] Quenching
Hi there my fellow
IHC reaction.
Go to the DAKO website and download a free IHC manual that can explain you this
and any other aspect of the IHC general procedure.
René J.
--- On Wed, 6/29/11, Candice Smoots wrote:
From: Candice Smoots
Subject: [Histonet] Quenching
To: "Histonet"
Date: Wednesday, Jun
Hi there my fellow histonetters!
I was wondering if anyone out there can tell me the purpose of quenching? Thanks
I remain yours truely,
Candice Camille
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You wrote: I'm wondering how to quench auto-fluorescence in tissue from a
mouse that was treated with a FITC-labeled protein. What do people usually
use to quench the endogenous auto-fluorescence, and will this treatment
quench the FITC signal from the labeled protein?
Jennifer M. Anderson,
Good morning.
I'm wondering how to quench auto-fluorescence in tissue from a mouse
that was treated with a FITC-labeled protein. What do people usually
use to quench the endogenous auto-fluorescence, and will this treatment
quench the FITC signal from the labeled protein?
Thanks a lot for your h
