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On Tue, 9/23/14, Geoff wrote:
Subject: Re: [Histonet] RE: Artifact in IHC
To: histonet@lists.utsouthwestern.edu
Date: Tuesday, September 23, 2014, 3:40 AM
Teri makes an excellent
point about higher alcohols causing
precipitation of phosphate salts.
Geoff
On 9/22/2014 1:1
Teri makes an excellent point about higher alcohols causing
precipitation of phosphate salts.
Geoff
On 9/22/2014 1:17 PM, Teri Johnson wrote:
Hi Donna,
Interesting conundrum! A few questions:
- Do you see it in tissues that have not undergone any staining, just floated
in buffer and covers
Hi Donna,
Interesting conundrum! A few questions:
- Do you see it in tissues that have not undergone any staining, just floated
in buffer and coverslipped?
- Have you tried wet mounting them after staining and prior to dehydration and
clearing to see if the artifact is there?
- Have you recen
