Cheri Miller
To: "Sherwood, Margaret" ; "Breeden, Sara"
; "Mahoney, Janice A" ;
histonet ;
"histonet-boun...@lists.utsouthwestern.edu"
Sent: Fri, January 15, 2010 11:14:30 AM
Subject: RE: [Histonet] RE: Eosin evaporation.
We do this as well, that is why I am
,Janice A; Cheri Miller; histonet;
histonet-boun...@lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: Eosin evaporation.
I agree Sally; we cover as soon as the run is over and had to adjust the level
of our H & E since the frosted end of the slides were getting stained as well.
We also tr
boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara
Sent: Friday, January 15, 2010 10:22 AM
To: Mahoney,Janice A; Cheri Miller; histonet;
histonet-boun...@lists.utsouthwestern.edu
Subject: RE: [Histonet] RE: Eosin evaporation.
Eosin is alcohol-based,
Eosin is alcohol-based, thus the evaporation. The containers on my
autostainer get covered as soon as the last run is finished and that
slows evaporation somewhat but I do replenish as needed. I like my eosin
level in the container a little lower so we don't have pink-stained
frosted ends, but that
Hi Cheryl,
Maybe the auto stainer does not drain the rack a well or it could just be a
matter of the dipping and dunking that is causing more evaporation.
Jan
Omaha, NÉE
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