Ceri, There are several ways to darken the eosin counterstain: 1. Stain longer in eosin, though with some eosins including eosin Y, this might not necessarily darken the staining since the eosin Y is a yellow red, eosin B seems to be more bluish red 2. Under-differentiate the haematoxylin, though this can result in overly stained nuclei (especially those of plasma cells). 3. Include a darker red dye in your eosin stain eg Erythrosine or even phloxine (not quite as dark).
Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ceri Allen Sent: Wednesday, 23 January 2013 8:58 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Eosin intensity Dear All, Please can I ask what everyone uses as a standard Eosin recipe? The new pathologist in our department has asked for the stain to be darker, 'like the hospital does it'. I work on the research side of a vet school, so I'm not sure what histology departments in hospitals normally use. Many thanks Ceri Research Technician School of Veterinary Medicine and Science University of Nottingham This message and any attachment are intended solely for the addressee and may contain confidential information. If you have received this message in error, please send it back to me, and immediately delete it. Please do not use, copy or disclose the information contained in this message or in any attachment. Any views or opinions expressed by the author of this email do not necessarily reflect the views of the University of Nottingham. This message has been checked for viruses but the contents of an attachment may still contain software viruses which could damage your computer system: you are advised to perform your own checks. Email communications with the University of Nottingham may be monitored as permitted by UK legislation. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet