Try Neg-50 (Fisher, cat#22-110-617) instead of OCT, we don't have any problem with fresh-frozen mouse eyes.
Anatoli Gleiberman, PhD Director of Histopathology Cleveland Biolabs, Inc 73 High Street Buffalo, NY 14203 phone:716-849-6810 ext.354 fax:716-849-6817 e-mail: agleiber...@cbiolabs.com -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Boyce, Bobbie Sent: Monday, July 25, 2011 10:31 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Frozen eye problems Hello Histoneters, I'm having problems getting a good section on paraformaldyehyde fixed adult mouse eyes frozen in TBS tissue freezing medium. One of the problems was the orentation of them. They were too close to the edge. I also, wanted to try a harder freezing medium, so I corrected oriented and used an OCT equivalent tissue freezing medium. They looked better, but still not as nice as I think they should be. My eye contact tells me that they should be cut at -20 for adult and -14 for neonatal. They have me set the knife angle at 0 to -2.5. I also tried cutting these as I would cut any other frozen issue, but I'm just pulling my hair out. Does anyone have any other suggestions that I might try? Your help would be greatly appreciated. Bobbie Boyce Histology Specialist III duPont Hospital for Children Wilmington, DE 302-651-6771 (Lab) 302-651-5010 (Fax) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This communication may contain privileged information. It is intended solely for the use of the addressee. If you are not the intended recipient, you are strictly prohibited from disclosing, copying, distributing or using any of this information. If you received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. This communication may contain nonpublic information about individuals and businesses subject to the restrictions of the Gramm-Leach-Bliley Act. You may not directly or indirectly reuse or redisclose such information for any purpose other than to provide the services for which you are receiving the information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
