The95 for HIER is in liquid, The 82in the oven is dry slides. While wet high temps enhance HIER, It seems the high dry temp does harm epitopes (there is a paper somewhere on this but I don't have access to it right now).
Tim Morken Supervisor, Histology, Electron Microscopy and Neuromuscular Special Studies UC San Francisco Medical Center San Francisco, CA CONFIDENTIALITY NOTICE: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential, proprietary, and/or privileged information protected by law. If you are not the intended recipient, you may not use, copy, or distribute this email message or its attachments. If you believe you have received this email message in error, please contact the sender by reply email and destroy all copies of the original message. -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Preiszner, Johanna Sent: Monday, April 20, 2015 8:47 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC and oven temperature Hi Netters, is there something wrong with this logic: "If the tissue needs 95C for HIER, it's ok to dry the slides in an 82C oven." Of course I'll test it before I try it on real specimens, but maybe someone else already knows the answer... Thanks! Hanna Preiszner ETSU/QCOM _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
