I have place the deparaffinized slides into 10% neutral buffered formalin for
about 30 minutes before the heat retrieval. Somehow this seems to work
You can also lower the temperature of your retrieval and lengthen the time.
Good Luck.
Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervi
Sara
If you can you want to stick with an enzyme digestion such as proteinase K or
another enzyme. If the antibody requires HIER and will not work with enzyme
digestion then modify your retreival time and temp, we use 70C for 2 hours and
find that this works for most antibodies. Some antibodi
