> -Original Message-
> From: histonet-boun...@lists.utsouthwestern.edu [mailto:
> histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sue Hunter
> Sent: Wednesday, April 03, 2013 10:55 AM
> To: Sarah Dysart; histonet@lists.utsouthwestern.edu
> Subject: [Histonet] RE: Out of my comfort zone.
...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sue Hunter
Sent: Wednesday, April 03, 2013 10:55 AM
To: Sarah Dysart; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Out of my comfort zone...
Hello
Just wanted to add one more thing - we actually use a
Hello
Just wanted to add one more thing - we actually use a dedicated pyrex dish
(maybe 6x10 inches) for our water bath for RNA sections. We use warm tap
water, but you can put it in the microwave for a short bit if it needs to be
warmer. You can spray the dish with RNAse away and wipe before
Sarah
The proteinase K does a lot more than break the formalin linkages. To isolate
the RNA or DNA you have to break up the cell membranes, nuclear membranes and
all the other proteins in the cellular matrix to isolate the nucleic acids. I
don't think an antigen retrieval solution will do any
