Jennifer MacDonald asked: >>At a local lab when a pathologist orders a
PAS diastase the histotechnicians do just one slide with diastase.
They do not do an undigested slide. How would the pathologist know if
the digested slide had a glycogen to begin with? Am I over thinking
this?<<
If I want an u
It depends on the tissue-component to be shown. If you just want to see
tissue-architecture or eg. in liver mallory-bodies and glycogen doesn't play
a role, one slide would be sufficient.
Gudrun
On 3/15/2012 1:47 AM, Jennifer MacDonald wrote:
> Hi All,
> At a local lab when a pathologist orders
I disagree that two patient slides are needed. What is needed for diagnosis is
one patient slide with digestion, and two control slides, one with and one
without. If the control slide shows adequate digestion of the glycogen and the
other control slide shows adequate PAS positivity, the patholog
I'm hoping that the attachment will attach. It's an old procedure from the
1950's
Vikki
If this does not attach, please let me know.
http://jcs.biologists.org/content/s3-97/37/11.full.pdf
On Tue, Jun 28, 2011 at 10:47 AM, Elizabeth Chlipala wrote:
> Chromic acid will work but I think that's
Chromic acid will work but I think that's for PAS for fungus.
Liz
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308-1592
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
www.premierlab.com
Ship to address:
1567 Skyway Drive, Unit
Mirna,
Lee Luna (Histologic 6(1):77, 1976) suggests that the best control for PAS
staining, are the walls of blood vessels. They will show a bright red reaction
only if the technique is working well. Glycogen may stain well even with
sub-optimal reagents.
Regards
Tony Henwood JP, MSc, BAppSc,
We wash after Schiff's for 10 minutes in regular temp tap water. Don't know if
that helps or not..
Jeanine Bartlett
Infectious Diseases Pathology Branch
(404) 639-3590
jeanine.bartl...@cdc.hhs.gov
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