RE: [Histonet] RE: Re: Methanol in H2O2 explanation

2012-07-11 Thread Connolly, Brett M
ts.utsouthwestern.edu] On Behalf Of Geoff McAuliffe Sent: Wednesday, July 11, 2012 12:50 PM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] RE: Re: Methanol in H2O2 explanation I agree, lots of "lore" gets passed down without question or understanding. Many years ago a new

Re: [Histonet] RE: Re: Methanol in H2O2 explanation

2012-07-11 Thread Geoff McAuliffe
lists.utsouthwestern.edu] On Behalf Of Gudrun Lang Sent: Wednesday, July 11, 2012 7:17 AM To: histonet@lists.utsouthwestern.edu Subject: AW: [Histonet] RE: Re: Methanol in H2O2 explanation For the enzymatic activity of peroxidase it needs an electron-donator (or receptor - I can't find the

RE: [Histonet] RE: Re: Methanol in H2O2 explanation

2012-07-11 Thread Morken, Timothy
al Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Gudrun Lang Sent: Wednesday, July 11, 2012 7:17 AM To: histonet@lists.utsouthwestern.edu Subject: AW: [Histonet] RE: Re: Methanol in H2O2 explanation For the enzymatic activity of pero

[Histonet] RE: Re: Methanol in H2O2 explanation

2012-07-11 Thread Bea DeBrosse-Serra
And the reason for that is...??? Beatrice DeBrosse-Serra HT(ASCP)QIHC Isis Pharmaceuticals Antisense Drug Discovery 2855 Gazelle Ct. Carlsbad, CA 92010 760-603-2371 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu]

AW: [Histonet] RE: Re: Methanol in H2O2 explanation

2012-07-11 Thread Gudrun Lang
tonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Tony Henwood (SCHN) Gesendet: Mittwoch, 11. Juli 2012 06:21 An: 'Hobbs, Carl'; histonet@lists.utsouthwestern.edu Betreff: [Histonet] RE: Re: Methanol in H2O2 explanation Hi Carl, What

[Histonet] RE: Re: Methanol in H2O2 explanation

2012-07-11 Thread Settembre, Dana
Carl, H202 mixed with Buffer here.(instead of aq.) That's OK too, right? Dana -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Hobbs, Carl Sent: Tuesday, July 10, 2012 11:32 PM To: histonet@lists.utsouthwest

[Histonet] RE: Re: Methanol in H2O2 explanation

2012-07-10 Thread Tony Henwood (SCHN)
Hi Carl, What do you mean by "Why do people rehydrate after dewaxing?" Do you really mean that slides do not require rehydration or do you mean that slides can be left to dry after de-waxing prior to staining. Re-hydration is necessary, otherwise xylene will prevent aqueous stains from doing t