I have found that the major problem with most of these enzymehistochemical stains is that the sections should not be allowed to sit too long at room temperature before staining. Either stain immediately on sectioning or store at -70oC. The enzymes tend to deteriorate quite rapidly at room temp.
Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Laboratory Manager & Senior Scientist Tel: 612 9845 3306 Fax: 612 9845 3318 the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Celebre Julia Sent: Saturday, 26 January 2013 6:01 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] SDH methods for muscles I give up!! One week it works, the next week it doesn't, same reagents, same method, same tech, for the life of me I can't figure out what is the problem. If anybody has a fail safe method they are willing to share, please send it along. I'm pretty sure my neuropathologist will appreciate all the help I can get. Julia Celebre Sr MLT Anatomic Pathology Hamilton General Hospital 905-527-4322 ext 46179 This information is directed in confidence solely to the person named above and may not otherwise be distributed, copied or disclosed. Therefore, this information should be considered strictly confidential. If you have received this email in error, please notify the sender immediately via a return email for further direction. Thank you for your assistance. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ********************************************************************************* This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses. ********************************************************************************* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
