Francie,
Conjugated antibodes CAN be detected by secondary antibodies. So the
conjugation doesn't really hide the primary antibody to the secondary.
I've done it and seen it with biotin and FITC labeled antibodies.
Something to consider..
Mark
On Wed, Nov 7, 2012 at 11:43 AM, Frances Elizabeth
Hi All,
Hope this day finds you well.
I have two antibodies in the lab that I would like to use together for ICC. One
is a mouse anti-human pre-conjugated antibody, the other is a mouse anti-human
unconjugated antibody. My question ishow to get these guys to play nice
with one another or s