Re: [Histonet] RE: recommended ICC protocol for use of Conjugated mouse antibodies with unconjugated mouse antibodies

2012-11-07 Thread Mark Tarango
Francie, Conjugated antibodes CAN be detected by secondary antibodies. So the conjugation doesn't really hide the primary antibody to the secondary. I've done it and seen it with biotin and FITC labeled antibodies. Something to consider.. Mark On Wed, Nov 7, 2012 at 11:43 AM, Frances Elizabeth

[Histonet] RE: recommended ICC protocol for use of Conjugated mouse antibodies with unconjugated mouse antibodies

2012-11-07 Thread Frances Elizabeth Barron
Hi All, Hope this day finds you well. I have two antibodies in the lab that I would like to use together for ICC. One is a mouse anti-human pre-conjugated antibody, the other is a mouse anti-human unconjugated antibody. My question ishow to get these guys to play nice with one another or s