Does anyone cut frozen RNAlater treated human tumor and normal tissues without
fixation? If yes, it will be possible to share the protocol?
Respectfully,
Sadeq Kharzai
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwest
Hello, Esteemed Histonetters!
I have a collaborator who asked me to drop his (mouse) bones into RNAlater for
RNA isolation, later. These animals were double labeled for histomorphometry as
well. He now wants to know if he can back them out of RNAlater and use them for
any histology-related prot
Has anyone successful fixed & embedded tissues in OCT after using
RNAlater buffer? I've found a paper that says this doesn't affect tissue
integrity but I need for info regarding protocol (To defrost? Fixative?
Timing? Temperature Etc) ...and more than anything feedback from someone
who has u
Dear Histonetters,
I'm looking for methods to preserve intestinal tissue samples, in order
to later isolate RNA from laser microdissected cryosections.
I'm a bit worried about the intestinal enzymes (not sure if the RNA will
be preserved even at -80C for very long). I was told that RNALater mig
