...@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda
[lbla...@digestivespecialists.com]
Sent: Wednesday, July 01, 2009 1:47 PM
To: 'Jennifer Johnson'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Re: Eosin in alcohol
We use about the same amount or eosin in the first 100% alcohol and also do
nifer Johnson
Subject: [Histonet] Re: Eosin in alcohol
I have never heard of eosin interfering with IHC, as a matter of fact, I did
use it regularly and never had a problem with the IHC.
Perhaps there was another cause for the problems with the IHC.
rené J.
--- On Wed, 7/1/09, Jennifer Johnson
: Eosin in alcohol
To: histonet@lists.utsouthwestern.edu
Date: Wednesday, July 1, 2009, 1:06 PM
We have used Eosin in the last 95% alcohol on the tissue processor for several
years. I usually add approximately 5 ml to the full jug. It is a great tool
to use for embedding. However, we
I think they are probably getting weak unwanted staining with P504s/AMACR
antibody on benign glands on their PIN4s.
Since this antibody is usually pink/red, it sounds like someone might have
decided that the unwanted color came from eosin in the processor, but this
would be the wrong conclusion.
ly 01, 2009 1:07 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Re: Eosin in alcohol
We have used Eosin in the last 95% alcohol on the tissue processor for several
years. I usually add approximately 5 ml to the full jug. It is a great tool
to use for embedding. However, we recei
ieval process of staining
immunos.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jennifer Johnson
Sent: Wednesday, July 01, 2009 1:07 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Re: Eos
Histologist, Southside Regional Medical
Center,
200 Medical Park Boulevard, Petersburg, Va. 23805, T: 804-765-5050, F:
804-765-5582, dkb...@chs.net
Jennifer Johnson
Sent by: histonet-boun...@lists.utsouthwestern.edu
07/01/2009 01:09 PM
To
cc
Subject
[Histonet] Re: Eosin in alcohol
We
We have used Eosin in the last 95% alcohol on the tissue processor for several
years. I usually add approximately 5 ml to the full jug. It is a great tool
to use for embedding. However, we received a letter from the lab that we send
our prostate biopsies to saying that it was undesirable bec
