Patrick,
We do a lot of frozen section IHC work. Years ago Gayle Callis turned me on to
fixing in cold acetone:ethanol (3:1) . We keep it at -20C and I fix for 10
min. on the bench then wash in PBS and proceed with the IHC. We do dry slides
for at least 30 min before fixing. This has worked w
-boun...@lists.utsouthwestern.edu] On Behalf Of Bob Richmond
Sent: Saturday, November 15, 2014 2:34 PM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Re: Acetone fixation
Sheree H (where?) asks:
>>Have questions about using acetone at the grossing counter. Does
>>anyone
have
Sheree H (where?) asks:
>>Have questions about using acetone at the grossing counter. Does anyone
have problems with it being carried over into the formalin on the
processor? We rinse, open cassette to let it dry completely and even change
the cassette. I've had issues with my processor and want t
Atoska Gentry asked:
hello, if any of you have acetone fixation incorporated into you frozen
section H& E staining protocol will you please advise me on adjustments
necessary for routine staining protocol. Also, out of curiosity please
enlighten me on the purpose for post sectioning acetone fixatio
What is your goal? A routine H&E or immuno'setc.??
>>> "Atoska Gentry" 3/25/2010 12:21 PM >>>
hello, if any of you have acetone fixation incorporated into you
frozen
section H& E staining protocol will you please advise me on
adjustments
necessary for routine staining protocol. Also, out of
hello, if any of you have acetone fixation incorporated into you frozen
section H& E staining protocol will you please advise me on adjustments
necessary for routine staining protocol. Also, out of curiosity please
enlighten me on the purpose for post sectioning acetone fixation on
tissue samples
