Carol Freeman asks about using eosin and other dyes to mark small
specimens for better recovery during embedding.
I've found safranin O to be the best of these. Use the Gram stain
counterstain used in microbiology. You mark the specimens directly, on
those blue biopsy pads. It doesn't dissolve out
Here is a paper I found:
Capillary Electrophoresis Artifact Due to Eosin
Journal of Molecular Diagnostics, Vo. 7, No. 1, February 2005
Our molecular people are studying this issue to see how big of a problem it is.
Tim Morken
Supervisor, Electron Microscopy
Department of Pathology
UC San Franci
We also add liquid eosin (~75mL) to our "dirtiest" 100% to help aide the sight
of the small biopsies for embedding. You are correct though, that eosin does
fluoresce.
I understand why you would be concerned about FISH, so wanted to share this. I
have heard from fellow techs in the field that u
We use eosin in our last 100% alcohol on the processor. It is a great help for
our person embedding to be able to see and orient the small biopsies. We have
been doing this for years and have never had any problem with the processor or
any subsequent staining that has been performed. We also
