Hello
My experience is that most Immunofluorescence dyes do not withstand alcohol
and organic solvents dehydration.
You can use aqueous mounting medium with anti-fading agent.
Frozen brain tissue is tough to work with; since it contains tons of
lipids.
Lots of mounting mediums will produce air bub
Sorry, I should have mentioned that this will be done on thick frozen
sections.
Bob
On Mon, Jul 20, 2009 at 10:37 AM, Bob Nienhuis wrote:
> Will the Alexa Fluor dyes withstand some dehydration and clearing in
> alcohol and xylene?
>
> If they won't are there any bright fluorescent dyes that can
