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On Jun 29, 2013, at 12:03 PM, "histonet-requ...@lists.utsouthwestern.edu" 
<histonet-requ...@lists.utsouthwestern.edu> wrote:

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> Today's Topics:
> 
>   1. Decalcifier, adding Zinc Fixative (M.O.)
>   2. RE: Disposable blade information (Tom McNemar)
>   3. RE: Dragon users  (Gray, Ed)
>   4. PowerPath Issues (Willis, Donna G.)
>   5. Re: PowerPath Issues (Jesus Ellin)
>   6. Alport Syndrome (CHRISTIE GOWAN)
>   7. antibody providers for cytokine/chemokine IHC (Nathan Ostlie (S))
> 
> 
> ----------------------------------------------------------------------
> 
> Message: 1
> Date: Fri, 28 Jun 2013 11:09:04 -0700
> From: "M.O." <modz9...@gmail.com>
> Subject: [Histonet] Decalcifier, adding Zinc Fixative
> To: histonet@lists.utsouthwestern.edu
> Message-ID:
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> 
> Hello Histonet!  Would anyone know if it is okay to add zinc fixative to
> TBD-2 decalcifier?  What ratio would be okay?
> 
> I am having an issue with human bone samples and want to make sure that
> these bones are properly fixed.  They are about 7mm thick and I am fixing
> them for 4 days, but additional fixation in the TBD-2 would be best.
> 
> Thank you,
> Merissa
> 
> 
> ------------------------------
> 
> Message: 2
> Date: Fri, 28 Jun 2013 14:16:29 -0400
> From: Tom McNemar <tmcne...@lmhealth.org>
> Subject: [Histonet] RE: Disposable blade information
> To: "'Hannen, Valerie'" <valerie.han...@parrishmed.com>,
>    "'histonet@lists.utsouthwestern.edu'"
>    <histonet@lists.utsouthwestern.edu>
> Message-ID:
>    <E9A90E28259D2F4E84308C5E8EA8F7B40153AB2F8FB2@lmhs-exchange>
> Content-Type: text/plain; charset="us-ascii"
> 
> I didn't think anybody still used steel knives....  I remember those days....
> 
> I would say to get away from the steel as soon as you can.  Disposable will 
> make your life so much easier.
> 
> As you already know, it is near impossible to give you anything more than a 
> guestimate but I would imagine that you could get by with one to three per 
> day depending on case mix, staples, sutures, calcifications, etc.  There are 
> so many variables.  It also depends on how many people are cutting those 50 
> blocks.
> 
> There are a lot blades to choose from.  You could probably get by for quite 
> awhile just trying out different samples....
> 
> See if you can find a rep that can loan you a blade holder and try some.  The 
> holder will be the biggest upfront expense.
> 
> I don't really know what the difference is between the high and low profiles 
> but I suspect not much more than the height.
> 
> I have tried many different brands over the years and always go back to the 
> AccuEdge low profile.
> 
> Tom McNemar, HT(ASCP)
> Histology Co-ordinator
> Licking Memorial Health Systems
> (740) 348-4163
> (740) 348-4166
> tmcne...@lmhealth.org
> www.LMHealth.org
> 
> 
> -----Original Message-----
> From: histonet-boun...@lists.utsouthwestern.edu 
> [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Hannen, 
> Valerie
> Sent: Friday, June 28, 2013 12:03 PM
> To: 'histonet@lists.utsouthwestern.edu'
> Subject: [Histonet] Disposable blade information
> 
> Good Morning all,
> 
> I am having to do a cost analysis study for the cost effectiveness of 
> Sharpening steel blades vs. transitioning to disposable blades. I know...what 
> you all are thinking..
> 
> "Your're still using steel blades??"...Yes, we are... :).  Our current knife 
> sharpener has some issues, and I have decided to weigh buying a used knife 
> sharpener against
> 
> switching to the disposable system.  So... a little background for you. We 
> are a relatively small department, we average @ 50 blocks a day, of various 
> tissues types.
> 
> My questions to you are this...and I know that answers will only be  
> estimations...but it is  a place for me to start.
> 
> What would be the average number of disposable blades that I would probably 
> use in a day/ week?  On an average...how many blocks can be cut on each blade?
> 
> Has anyone recently made this transitiion? If so, what were some of the 
> challenges??   I'm going to show my lack of knowledge of disposable blades 
> with this next
> 
> question...but here it goes.. What is the difference between a low profile 
> blade and a high profile blade...and would I need to buy both??
> 
> Ok... my head is hurting...so.. I am going to leave you with all the above 
> questions.
> 
> Any and all information, suggestions, etc. will be greatly appreciated!!
> 
>   Thanks and have a GREAT day!!
> 
> Valerie A. Hannen, MLT(ASCP),HTL,SU(FL)
> Histology Section Chief
> Parrish Medical Center
> 951 N. Washington Ave.
> Titusville, Florida 32976
> Phone:(321) 268-6333 ext. 7506
> Fax: (321) 268-6149
> valerie.han...@parrishmed.com
> 
> 
> 
> 
> 
> 
> 
> 
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> 
> ------------------------------
> 
> Message: 3
> Date: Fri, 28 Jun 2013 14:15:21 -0400
> From: "Gray, Ed" <eg...@hsc.wvu.edu>
> Subject: [Histonet] RE: Dragon users 
> To: "histonet@lists.utsouthwestern.edu"
>    <histonet@lists.utsouthwestern.edu>
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>    <0BBA94171603A6468FFBA03A45A029FC04441004B7@MSEXCL.hscresex.local>
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> 
> We use Sennheiser RF sets in the gross room.  The sound quality is excellent 
> and produce the best recognition of any wireless mike we've tried.  These 
> were pretty much the only wireless option Nuance would recommend when we 
> first started using Dragon several years ago. The cons are:
> 1.  The RF receiver is 21 x 10.5 x 3.5 cm with an external antenna and power 
> supply, not huge but significant compared to a USB Bluetooth adapter.
> 2.  You are wired to a transmitter which is not terribly large or heavy but a 
> wire to your belt/pocket none-the-less.
> 3.  They are battery hogs and quality drops rapidly once the indicator goes 
> below 50%.
> 4.  They were expensive when we bought them.  I think they were over $300.00. 
>  We bought some replacement headsets at one point...also expensive.  They 
> have held up very well though.
> 
> 
> 
> Ed Gray | Pathology IT Analyst | Phone: 304-293-2945 | Fax 304-293-1627 | WVU 
> Healthcare l eg...@wvuhealthcare.com
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> ------------------------------
> 
> Message: 3
> Date: Thu, 27 Jun 2013 19:23:06 +0000
> From: Nancy Schmitt <nancy_schm...@pa-ucl.com>
> Subject: [Histonet] Dragon users
> To: "Histonet (histonet@lists.utsouthwestern.edu)"
>    <histonet@lists.utsouthwestern.edu>
> Message-ID:
>    <906b4da90ed1db4db6c7e94d7cee6c36813d5...@peitha.wad.pa-ucl.com>
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> 
> Hi All-
> 
> we are using Dragon Enterprise and currently using Plantronics wireless 
> headsets - does anyone have any experience with other wireless microphone 
> options?
> 
> Thanks for your input-
> 
> NancySchmitt
> 
> Dubuque, IA
> 
> 
> 
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> 
> 
> ------------------------------
> 
> Message: 4
> Date: Fri, 28 Jun 2013 16:25:05 +0000
> From: "Willis, Donna G." <donna.wil...@baylorhealth.edu>
> Subject: [Histonet] PowerPath Issues
> To: "histonet@lists.utsouthwestern.edu"
>    <histonet@lists.utsouthwestern.edu>
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> 
> Anyone out there in Histoland having issues with PowerPath (Sunquest) 
> customer service?
> 
> Thanks,
> 
> Donna Willis, HT/HTL (ASCP)
> Anatomic Pathology Manager
> Baylor University Medical Center-Dallas
> ph. 214-820-2465 office
> ph. 214-725-6184 mobile
> donna.wil...@baylorhealth.edu
> 
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> 
> ------------------------------
> 
> Message: 5
> Date: Fri, 28 Jun 2013 19:01:19 +0000
> From: Jesus Ellin <jel...@yumaregional.org>
> Subject: Re: [Histonet] PowerPath Issues
> To: "Willis, Donna G." <donna.wil...@baylorhealth.edu>
> Cc: "histonet@lists.utsouthwestern.edu"
>    <histonet@lists.utsouthwestern.edu>,    Debbie Balli
>    <debbie.ba...@elekta.com>
> Message-ID: <a6d607c5-aae0-4552-89cb-467eb8f35...@yumaregional.org>
> Content-Type: text/plain; charset="us-ascii"
> 
> Donna this is Jesus Ellin and I am the Sunquest POwerPath Chair,, I am cc 
> Debbie Balli on this,, how can we help?
> 
> Sent from my iPad
> 
> On Jun 28, 2013, at 1:58 PM, "Willis, Donna G." 
> <donna.wil...@baylorhealth.edu> wrote:
> 
>> Anyone out there in Histoland having issues with PowerPath (Sunquest) 
>> customer service?
>> 
>> Thanks,
>> 
>> Donna Willis, HT/HTL (ASCP)
>> Anatomic Pathology Manager
>> Baylor University Medical Center-Dallas
>> ph. 214-820-2465 office
>> ph. 214-725-6184 mobile
>> donna.wil...@baylorhealth.edu
>> 
>> **********************************************************************
>> This e-mail may contain confidential and/or privileged information. This 
>> information is intended only for the use of the individual(s) and 
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>> further disclosures are prohibited without proper authorization. If you are 
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>> notify the sender immediately and destroy this e-mail. Any unauthorized 
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>> strictly forbidden and possibly a violation of federal or state law and 
>> regulations. Baylor Health Care System, its subsidiaries, and affiliates 
>> hereby claim all applicable privileges related to this information.
>> _______________________________________________
>> Histonet mailing list
>> Histonet@lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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> 
> ------------------------------
> 
> Message: 6
> Date: Fri, 28 Jun 2013 19:36:46 +0000
> From: CHRISTIE GOWAN <christiego...@msn.com>
> Subject: [Histonet] Alport Syndrome
> To: "histonet@lists.utsouthwestern.edu"
>    <histonet@lists.utsouthwestern.edu>
> Message-ID: <blu173-w1098bfa29feb1b10cda647ae...@phx.gbl>
> Content-Type: text/plain; charset="iso-8859-1"
> 
> Is anyone using the Fluorchrome-Conjugated MoAbs for Alport Syndrome 
> distributed by Cosmo Bio Co., LTD.?
> 
> We are using it on cryostat sections of renal tissue and the Texas Red 
> fluorochrome seems to be extremely robust while the FITC-conjugated-anti 
> alpha2(IV) is weak. I would be interested to hear your outcomes/solutions. 
> 
> 
> 
> Christie Gowan HT (ASCP)
> UAB Hospital Surgical Pathology
> Supervisor Lab Services
> 205-934-4991
> cgo...@uabmc.edu
> 
>                         
> 
> ------------------------------
> 
> Message: 7
> Date: Fri, 28 Jun 2013 20:17:18 +0000
> From: "Nathan Ostlie (S)" <nathan.ost...@coloradocollege.edu>
> Subject: [Histonet] antibody providers for cytokine/chemokine IHC
> To: "histonet@lists.utsouthwestern.edu"
>    <histonet@lists.utsouthwestern.edu>
> Message-ID:
>    <8b449980f6f94ef684bd9dc45c3c7...@by2pr06mb012.namprd06.prod.outlook.com>
>    
> Content-Type: text/plain; charset="us-ascii"
> 
> Hello,
> 
> I am new to immunohistochemistry and I am in the process of ordering primary 
> antibody for cytokine/chemokine staining. Does anyone have recommendations 
> for companies that sell antibody compatible with IHC?
> 
> Examples of cytokines/chemokines we're hoping to stain for: CXCL5, CCL2, 
> CXCL10, IL-6, IL-17 IFN-g
> 
> Thanks, Nate
> 
> 
> ------------------------------
> 
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> End of Histonet Digest, Vol 115, Issue 32
> *****************************************

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