Barb, Eric, all- We also use a diamond pen/pencil-and they do contain an industrial grade diamond, so they are quite reasonably priced; I looked one up on the VWR website (just as a reference, not necessarily an endorsement! :o) and they run about $20-30 each. They last a very long time, too-We¹ve had ours for several years.
We use them for our direct IFs, FISH, anything dark field. Stephanie Stephanie Rodriguez, HTL(ASCP), QIHC Lead Molecular Technologist-FISH IHC Technologist III Phenopath Laboratories Seattle, WA (206) 374-9000 On 10/5/10 10:15 AM, "histonet-requ...@lists.utsouthwestern.edu" <histonet-requ...@lists.utsouthwestern.edu> wrote: > Message: 11 > Date: Tue, 5 Oct 2010 10:53:36 -0400 > From: "Gagnon, Eric" <gagn...@kgh.kari.net> > Subject: [Histonet] Direct immunofluorescence question > To: <histonet@lists.utsouthwestern.edu> > Message-ID: > <f93bd6329fc3ae4c8db116b985fbc31327c3a...@kghmail.kgh.on.ca> > Content-Type: text/plain; charset="iso-8859-1" > > Barb, > > Have you tried a diamond pencil? Available from a variety of sources, these > "pencils" can be used to etch a circle or other shape around the tissue to be > viewed. I believe they use lesser-quality industrial diamonds to make the > tips on the pencils (i.e. not the diamond ring quality). Coming across the > etched line under the fluorescent microscope produces brightness that helps > the pathologist find the tissue. > > We circle our DIF's on the back of the slide - it won't wash off and won't > interfere with reagents this way, but is still visible in a darkened room. > > Hope this helps, > > Eric Gagnon MLT > Histology Laboratory > Kingston General Hospital > Kingston, Ontario, Canada ____________________________________________________________________________ _________________________________________ Message: 1 Date: Mon, 4 Oct 2010 12:49:44 -0500 From: ba...@gundluth.org Subject: [Histonet] Direct immunofluoresent question To: histonet@lists.utsouthwestern.edu Message-ID: <of9419a09e.870a453a-on862577b2.00614e6b-862577b2.0061e...@gundluth.org> Content-Type: text/plain; charset=US-ASCII Hi all - The pathologist that reads our direct IF slides likes to have the sections of tissue circled on the slide so that they are easier to find. We currently use black marker on the back of the slide, but find that many times it smears and are looking for a better solution. What kind of slides do others use, and if you circle your sections what marker/pen/etcher do you use? Any thoughts are greatly appreciated! Barb Moe Gundersen Lutheran Medical Center La Crosse WI This e-mail message, including any attachments, is for the sole use of the intended recipients and may contain privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by e-mail and destroy all copies of the original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A. at (206) 374-9000. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet