RE: [Histonet] Re: Histonet Digest, Vol 97, Issue 21

2011-12-19 Thread gayle callis
...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Madeleine Huey Sent: Sunday, December 18, 2011 9:19 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Histonet Digest, Vol 97, Issue 21 Colleen, Many years ago I used Pharmingen's ant

[Histonet] Re: Histonet Digest, Vol 97, Issue 21

2011-12-18 Thread Madeleine Huey
Colleen, Many years ago I used Pharmingen's anti-Rat CD31, clone TLD-3A12 (I think, you need to check it out if they still sell it). This antibody is not recommend for formalin fixed paraffin tissues, but it will work. Following steps are critical; 1) Do not use HIER, use enzymatic digestion (n