...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Madeleine Huey
Sent: Sunday, December 18, 2011 9:19 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Re: Histonet Digest, Vol 97, Issue 21
Colleen,
Many years ago I used Pharmingen's ant
Colleen,
Many years ago I used Pharmingen's anti-Rat CD31, clone TLD-3A12 (I
think, you need to check it out if they still sell it).
This antibody is not recommend for formalin fixed paraffin tissues,
but it will work.
Following steps are critical;
1) Do not use HIER, use enzymatic digestion (n