Imho....just do it on your tissue and include a no primary control. If the latter is clear, don't worry about MOM. If it is not, then we can advise further. You don't state what Ab/tissue/detection system you are using...... This would help a lot as protocols will vary according to the tissue used . I never want to use a more complicated detection system than I have to, just because it is MOM. Occam's razor! carl
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