; histonet@lists.utsouthwestern.edu
Cc: itai.mo...@mail.huji.ac.il
Subject: Re: [Histonet] Re: Masson's trichrom - problem with nuclei staining
I do Weigert's staining for 10 minutes and use it for a week at the most.
I,
too, have heard conflicting times about the stability of the Weigert's, b
evious message: **[Histonet] Re: Masson's trichrom - problem with
nuclei
staining*<http://lists.utsouthwestern.edu/mailman/htdig/histonet/2010-September/052630.html>
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Subject: Re: [Histonet] Re: Masson's trichrom - problem with nuclei
staining
Hi, and thank's for the quick replay.
I'm using a fresh Weigert's prepared from the sigma kit ever
--
>> *From:* Andrea Marion
>>
>> *To:* histonet@lists.utsouthwestern.edu
>> *Cc:* itai.mo...@mail.huji.ac.il
>> *Sent:* Fri, September 17, 2010 10:31:16 AM
>> *Subject:* [Histonet] Re: Masson's trichrom - problem with nuclei
>> staining
>&
sts.utsouthwestern.edu
> *Cc:* itai.mo...@mail.huji.ac.il
> *Sent:* Fri, September 17, 2010 10:31:16 AM
> *Subject:* [Histonet] Re: Masson's trichrom - problem with nuclei staining
>
> Hi Itai,
>
> I am interested to hear if you've resolved this problem. We use the same
rea Marion
To: histonet@lists.utsouthwestern.edu
Cc: itai.mo...@mail.huji.ac.il
Sent: Fri, September 17, 2010 10:31:16 AM
Subject: [Histonet] Re: Masson's trichrom - problem with nuclei staining
Hi Itai,
I am interested to hear if you've resolved this problem. We use the same
kit to s
Hi Itai,
I am interested to hear if you've resolved this problem. We use the same
kit to stain mouse heart and embryo sections, PFA fixed. Our protocol is
similar to the one you mentioned. I cannot get nuclei staining with this
method either. The nuclei are well stained (ie black) up to the PMA/P
