[Histonet] Re: PAS Diastase

2012-03-16 Thread Bob Richmond
Jennifer MacDonald asked: >>At a local lab when a pathologist orders a PAS diastase the histotechnicians do just one slide with diastase. They do not do an undigested slide. How would the pathologist know if the digested slide had a glycogen to begin with? Am I over thinking this?<< If I want an u

AW: [Histonet] Re: PAS Diastase

2012-03-15 Thread Gudrun Lang
It depends on the tissue-component to be shown. If you just want to see tissue-architecture or eg. in liver mallory-bodies and glycogen doesn't play a role, one slide would be sufficient. Gudrun On 3/15/2012 1:47 AM, Jennifer MacDonald wrote: > Hi All, > At a local lab when a pathologist orders

[Histonet] Re: PAS Diastase

2012-03-15 Thread Theresa (Teri) Johnson
I disagree that two patient slides are needed. What is needed for diagnosis is one patient slide with digestion, and two control slides, one with and one without. If the control slide shows adequate digestion of the glycogen and the other control slide shows adequate PAS positivity, the patholog