Thanks Gayle for the tip, wouldn't hurt to improve. I will test it and post the results.
THANK YOU, PATTI RUBEN-NELSON H.T.(ASCP) PNP LABORATORY CONSULTANTS SUPERVISOR/DGC P.O. BOX 412 CABAZON, CA. 92230 cell (909) 841-9761 nelsonr...@verizon.net CONFIDENTIALITY NOTICE: This message and any included attachments are from Patti Nelson, PNP Laboratory Consultants and are intended only for the addressee. The information contained in this message is confidential and may contain privileged, confidential, proprietary and/or exemption from disclosure under applicable law. Unauthorized forwarding, printing, copying, distribution, or use of such information is strictly prohibited and may be unlawful. If you are not the addressee, please promptly delete this message and notify the sender of the delivery error by e-mail or you may call 909-841-9761. ________________________________ From: gayle callis <gayle.cal...@bresnan.net> To: nelsonr...@verizon.net Sent: Tuesday, November 8, 2011 9:26 AM Subject: Paraffin temperatures You are frying your biopsies at 67C too. Keep you paraffin temperature no more than 60C, closer to melting point of paraffin. Whoever told you to use 84C was WRONG!!! That is hot beyond belief, and they did not know what they were doing. Gayle M. Callis HTL/HT/MT(ASCP) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet