Dear Kim
I attached the peroxidase block method via personal email which works for either frozen or FFPE sections. You must buy the correct, exact glucose from ICN as Sigma discontinued this. I included the original publication. You can also find this blocking method on the Vector website. This has been discussed on Histonet before but possibly not in the context of the original publication for eosinophils in frozen section in minimally fixed e.g. acetone. If the sections are FFPE, you may have to extend the time or stick to 60 minute incubation in the glucose oxidase/glucose mixture. We nicknamed this GLUOX Peroxidase Block. For frozens, we used 60 minutes incubation but later found a publication using 30 min blocking of endogenous peroxidase (ordinary and not eosinophils) in frozen sections. Timing is something you might play with to shorten the staining time. Theory is slow, continuous production of H2O2 removes/blocks peroxidase and pseudoperoxidases in tissues/cells. I had feedback that it worked on paraffin sections with stubborn endogenous peroxidase. Good luck Gayle Callis _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet