Hi, We use Tech One Biomedical Services, Inc. as a third party PM and repair service. They are very nice to work with and not excessive with their fees. We have set up PM's with them for a lot of our older equipment. Toll free number is 866-497-3033 or www.techoneweb.com . Try them for your repairs, we have no problems with them for the four years we have been dealing with them.
No trees were harmed in the sending of this email. However, many electrons were severely inconvenienced. Theresa A Oeler, BS Senior Research Histologist NSABP Pathology Laboratory Federal North Building 1307 Federal Street, Suite 303 Pittsburgh, PA 15212 412/359-8931 Of. 412/359-3239 Fx. theresa.oe...@nsabp.org -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: Wednesday, December 16, 2009 1:20 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 73, Issue 24 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. FW: Notch-Nodal Paper (Margaryan, Naira) 2. RE: Blade Dispenser Thingy (Bonner, Janet) 3. RE: Blade Dispenser Thingy (Maria Katleba) 4. Histology Jobs Outside the USA (Maria Katleba) 5. COX2 in mice (Bell, Pat) 6. Anti-Flag antibody (Rachel, Rivka (NIH/NEI) [E]) 7. Leica Bond (Liz Chlipala) 8. RE: Leica Bond (Debrosse-Serra, Beatrice) 9. Re: FW: Notch-Nodal Paper (John Kiernan) 10. any info on Glutathione S-Transferase Pi (GST Pi)? (Jennifer Campbell) 11. Equipment Repair (Yaskovich, Ruth A (NIH/NIDCR) [E]) ---------------------------------------------------------------------- Message: 1 Date: Tue, 15 Dec 2009 12:00:54 -0600 From: "Margaryan, Naira" <nmargar...@childrensmemorial.org> Subject: [Histonet] FW: Notch-Nodal Paper To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <c1ba93040c6b9a4a8d84878f93fec36a038eec0...@cmhexcc01mbx.childrensmemori al.org> Content-Type: text/plain; charset="us-ascii" Hi histonetters, I am looking for some dye to be used in vivo like cell tracker or similar might work for identifying live cells. Any suggestions are appreciated, Naira ------------------------------ Message: 2 Date: Tue, 15 Dec 2009 13:51:23 -0500 From: "Bonner, Janet" <janet.bon...@flhosp.org> Subject: RE: [Histonet] Blade Dispenser Thingy To: "Breeden, Sara" <sbree...@nmda.nmsu.edu>, histonet@lists.utsouthwestern.edu Message-ID: <5f31f38c96781a4fbe3196ebc22d47807f2...@fhosxchmb006.adventistcorp.net> Content-Type: text/plain; charset=iso-8859-1 I keep the dispenser in a small plastic bag that seals along with the desiccant packages they put in the original box with the blades, (and any other desiccant packages I can find - from coverslips, inks etc.) As long as you keep the blades sealed with the desiccant, you shouldn't have anymore problems. This works really well in the cryostat chamber, too! Janet ________________________________ From: histonet-boun...@lists.utsouthwestern.edu on behalf of Breeden, Sara Sent: Tue 12/15/2009 9:52 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Blade Dispenser Thingy I have Feather dispensers with blades that I have to pry out, which is probably not a good thing. I've tried freezing the dispenser, putting it in an oven for a while and whacking the darned thing on the floor (not necessarily all at the same time..). Have we had this discussion before and have we solved this problem? Help? Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= ------------------------------ Message: 3 Date: Tue, 15 Dec 2009 11:39:48 -0800 From: Maria Katleba <maria.katl...@stjoe.org> Subject: [Histonet] RE: Blade Dispenser Thingy To: "Bonner, Janet" <janet.bon...@flhosp.org>, "Breeden, Sara" <sbree...@nmda.nmsu.edu>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <bf297e3b9fa5a14f8a14af49fd1a561701fb774...@sjsnt-scmail03.stjoe.org> Content-Type: text/plain; charset="us-ascii" I use one drop of microtome oil and they slip right out. I mean don't go crazy with the oil either! Maria Katleba HT(ASCP) MS Napa CA -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bonner, Janet Sent: Tuesday, December 15, 2009 10:51 AM To: Breeden, Sara; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Blade Dispenser Thingy I keep the dispenser in a small plastic bag that seals along with the desiccant packages they put in the original box with the blades, (and any other desiccant packages I can find - from coverslips, inks etc.) As long as you keep the blades sealed with the desiccant, you shouldn't have anymore problems. This works really well in the cryostat chamber, too! Janet ________________________________ From: histonet-boun...@lists.utsouthwestern.edu on behalf of Breeden, Sara Sent: Tue 12/15/2009 9:52 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Blade Dispenser Thingy I have Feather dispensers with blades that I have to pry out, which is probably not a good thing. I've tried freezing the dispenser, putting it in an oven for a while and whacking the darned thing on the floor (not necessarily all at the same time..). Have we had this discussion before and have we solved this problem? Help? Sally Breeden, HT(ASCP) NM Dept. of Agriculture Veterinary Diagnostic Services PO Box 4700 Albuquerque, NM 87106 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ======================================================= The information contained in this message may be privileged and/or confidential and protected from disclosure. If the reader of this message is not the intended recipient or an employee or agent responsible for delivering this message to the intended recipient, you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. If you have received this communication in error, please notify the sender immediately by replying to this message and deleting the material from any computer. ======================================================= _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Notice from St. Joseph Health System: Please note that the information contained in this message may be privileged and confidential and protected from disclosure. ------------------------------ Message: 4 Date: Tue, 15 Dec 2009 12:05:00 -0800 From: Maria Katleba <maria.katl...@stjoe.org> Subject: [Histonet] Histology Jobs Outside the USA To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <bf297e3b9fa5a14f8a14af49fd1a561701fb774...@sjsnt-scmail03.stjoe.org> Content-Type: text/plain; charset="us-ascii" Anyone know where you can find Histology positions OUTSIDE the USA? I have had several colleagues ask... and I am actually interested in this information as well..... Any thoughts? Maria Katleba HT(ASCP), MS Pathology Dept. Mgr. Queen of the Valley Medical Center 707-294-9229 cell 707-252-4411 x3689 ________________________________ Notice from St. Joseph Health System: Please note that the information contained in this message may be privileged and confidential and protected from disclosure. ------------------------------ Message: 5 Date: Tue, 15 Dec 2009 15:00:18 -0700 From: "Bell, Pat" <pat.b...@ucdenver.edu> Subject: [Histonet] COX2 in mice To: "'histonet@lists.utsouthwestern.edu'" <histonet@lists.utsouthwestern.edu> Message-ID: <64db27005e2fd3439e88502d7a5c91218e0894a...@cortez.ucdenver.pvt> Content-Type: text/plain; charset="us-ascii" Does anyone know of a good antibody for COX2 that works in the mouse? Thank you, Pat Pat Bell HT(ASCP) University of Colorado, Denver Medical Oncology; MS 8117 12801 E 17th Ave. Aurora, Co. 80045 303-724-6077 pat.b...@ucdenver.edu<mailto:pat.b...@ucdenver.edu> ------------------------------ Message: 6 Date: Tue, 15 Dec 2009 17:23:00 -0500 From: "Rachel, Rivka (NIH/NEI) [E]" <rach...@nei.nih.gov> Subject: [Histonet] Anti-Flag antibody To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <c74d7674.128c6%rach...@mail.nih.gov> Content-Type: text/plain; charset="iso-8859-1" Does anyone know of a good rabbit anti-Flag antibody that can be used on transfected mouse cells? -- Rivka A. Rachel National Eye Institute ------------------------------ Message: 7 Date: Tue, 15 Dec 2009 16:21:12 -0700 From: "Liz Chlipala" <l...@premierlab.com> Subject: [Histonet] Leica Bond To: <histo...@pathology.swmed.edu> Message-ID: <ee33be5c905a3046a7ff8f58a64c8e4b100...@server.premierlab.local> Content-Type: text/plain; charset="US-ASCII" Hey everyone I have a question regarding the Leica Bond IHC stainer. We are currently evaluating it. Is it true that you can not use a protein block on the instrument and if you do you have to rinse afterwards. I know that a lot of labs do not use protein blocks anymore but we routinely use them here, we use a serum free protein block on all of our IHC slides. If there is anyone out there that is willing to give me some advice on this? Also if anyone wants to comment on the use or lack of use of protein block that would be greatly appreciated also. I'm beginning to think I may be old fashioned but I'm a bit concerned about not using protein block, OMG I hope I'm not turning into one of those techs who has been in the field so long that they have become resistant to change, yikes! Thanks in advance Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 ------------------------------ Message: 8 Date: Tue, 15 Dec 2009 15:29:58 -0800 From: "Debrosse-Serra, Beatrice" <beatrice.debrosse-se...@pfizer.com> Subject: RE: [Histonet] Leica Bond To: "Liz Chlipala" <l...@premierlab.com>, <histo...@pathology.swmed.edu> Message-ID: <a5ad5cf370eec64a93b5155b6739df32b66...@lajamrexm01.amer.pfizer.com> Content-Type: text/plain; charset="us-ascii" Hi Liz, We have Bonds and use protein blocks all the time. It isn't part of the kit, but you can use it in one of the open containers. Beatrice DeBrosse-Serra Pathology Scientist Pfizer Global Research & Development CB4, 2150 10646 Science Center Drive San Diego, CA 92121 Phone# 858-622-5986 Fax# 858-678-8290 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Liz Chlipala Sent: Tuesday, December 15, 2009 3:21 PM To: histo...@pathology.swmed.edu Subject: [Histonet] Leica Bond Hey everyone I have a question regarding the Leica Bond IHC stainer. We are currently evaluating it. Is it true that you can not use a protein block on the instrument and if you do you have to rinse afterwards. I know that a lot of labs do not use protein blocks anymore but we routinely use them here, we use a serum free protein block on all of our IHC slides. If there is anyone out there that is willing to give me some advice on this? Also if anyone wants to comment on the use or lack of use of protein block that would be greatly appreciated also. I'm beginning to think I may be old fashioned but I'm a bit concerned about not using protein block, OMG I hope I'm not turning into one of those techs who has been in the field so long that they have become resistant to change, yikes! Thanks in advance Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Wed, 16 Dec 2009 00:39:53 -0500 From: John Kiernan <jkier...@uwo.ca> Subject: Re: [Histonet] FW: Notch-Nodal Paper To: "Margaryan, Naira" <nmargar...@childrensmemorial.org> Cc: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <fc52ed8f6247c.4b282...@uwo.ca> Content-Type: text/plain; CHARSET=US-ASCII Dear Naira Margaryan, Please clarify "in vivo", which means "in alive ...". What is your "..."? Do you want to stain living cells in thin-layer tissue cultures, suspended cells (blood, haemolymph etc), or cells in whole organisms that are small enough to live under a coverslip (such as rotifers, little nematodes and protozoans)? For larger animals, in vivo microscopy is possible only at the surface (skin and other accessible surfaces) or where there are transparent media (the eye). Beware of adverts for fluorescent stains with catchy names! If you hope to be a career scientist you will run into the peer review process, which is severe and does not like results based on unexplained trade secrets. If you explain your question on histonet, you will get plenty of good advice, supplemented with peer-reviewed references that you can check. John Kiernan Anatomy, UWO London, Canada = = = ----- Original Message ----- From: "Margaryan, Naira" <nmargar...@childrensmemorial.org> Date: Tuesday, December 15, 2009 13:02 Subject: [Histonet] FW: Notch-Nodal Paper To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> > Hi histonetters, > > I am looking for some dye to be used in vivo like cell tracker > or similar might work for identifying live cells. > > Any suggestions are appreciated, > Naira > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 10 Date: Wed, 16 Dec 2009 08:20:29 -0800 From: "Jennifer Campbell" <jcampb...@vdxpathology.com> Subject: [Histonet] any info on Glutathione S-Transferase Pi (GST Pi)? To: <histonet@lists.utsouthwestern.edu> Message-ID: <5658cbdb9eae6545abe50d2563d81bf82fa...@vdxserver01.vdxpathology.local> Content-Type: text/plain; charset="us-ascii" Hi All, Has anyone ever used this antibody in FFPE mouse or rat tissue? How easy of an antibody is it to work with? Other comments? We may be doing this for an upcoming project and I just wanted to know what I could be getting myself into. Thanks! Jen Campbell ------------------------------ Message: 11 Date: Wed, 16 Dec 2009 12:03:29 -0500 From: "Yaskovich, Ruth A (NIH/NIDCR) [E]" <ryaskov...@dir.nidcr.nih.gov> Subject: [Histonet] Equipment Repair To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <edd146c8a3c60a4b8cfbed938898272d050f291...@nihmlbx01.nih.gov> Content-Type: text/plain; charset="us-ascii" Does anyone know of a company that repairs equipment in the Maryland area? I'm in Bethesda and my old Tissue-Tek embedding center won't hold the temperature. Thanks for any help, Ruth Yaskovich National Institutes of Health Bethesda, Maryland ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 73, Issue 24 **************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet