Recently our IHC vendor sent a tech to help us out with some weak staining affecting multiple antibodies. Several staining protocols were changed, including adjusting the incubation time and adding amplification. The results were great in the slides that I was shown.
The tech said that revalidation was unnecessary, since the changes to the protocol were considered "minor". However, I personally disagree, since we don't know how the change in the staining protocol could potentially affect staining of other tissues (especially tissues that would be expected to be negative). Before subjecting our lab to the cost and effort of revalidation, I'd like to get your opinion. For new antibodies, I usually validate using 10 cases expected to be positive and 10 cases expected to be negative for the antibody in question. For revalidation, I was thinking of running 5 positive cases and 5 negative cases. Thanks for your input - Kevin Kurtz, M.D. St. Mary's Hospital Madison, Wisconsin Confidentiality Notice: This email message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet